Fabrication of Biotinylated Gradient Surface Based on the Micropatterned Polymer Brushes

Hai-li Zhao; Jin Sha; Lin-sheng Xie; Yu-lu Ma; Tao Chen

doi:10.11777/j.issn1000-3304.2021.21386

您当前的位置：

首页 >

文章列表页 >

Fabrication of Biotinylated Gradient Surface Based on the Micropatterned Polymer Brushes

Research Article | 更新时间：2024-10-08

- Fabrication of Biotinylated Gradient Surface Based on the Micropatterned Polymer Brushes
  增强出版
- ACTA POLYMERICA SINICA Vol. 53, Issue 6, Pages: 636-644(2022)
- 作者机构：
  
  1.昆明理工大学化学工程学院昆明 650504
  2.华东理工大学机械与动力工程学院上海 200237
- 作者简介：
  
  Jin Sha, E-mail: sjin@ecust.edu.cn
  Tao Chen, E-mail: chentao716@163.com
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2021.21386
  CLC：
- Published：20 June 2022，
  
  Published Online：08 March 2022，
  
  Received：15 December 2021，
  
  Accepted：24 January 2022
- 稿件说明：
移动端阅览
赵海利,沙金,谢林生等.基于聚合物刷微图案的生物素化梯度表面制备研究[J].高分子学报,2022,53(06):636-644.

Zhao Hai-li,Sha Jin,Xie Lin-sheng,et al.Fabrication of Biotinylated Gradient Surface Based on the Micropatterned Polymer Brushes[J].ACTA POLYMERICA SINICA,2022,53(06):636-644.
赵海利,沙金,谢林生等.基于聚合物刷微图案的生物素化梯度表面制备研究[J].高分子学报,2022,53(06):636-644. DOI： 10.11777/j.issn1000-3304.2021.21386.

Zhao Hai-li,Sha Jin,Xie Lin-sheng,et al.Fabrication of Biotinylated Gradient Surface Based on the Micropatterned Polymer Brushes[J].ACTA POLYMERICA SINICA,2022,53(06):636-644. DOI： 10.11777/j.issn1000-3304.2021.21386.

摘要

提出了基于叠氮功能化聚合物刷微图案制备生物素化梯度表面的方法. 通过数字微镜器件(DMD)调控光辐照引发表面原子转移自由基聚合(ATRP)反应，制备叠氮功能化的聚(2-(2-叠氮-2-甲基丙氧基)甲基丙烯酸乙酯)(PAMEMA)聚合物刷微图案，采用X射线光电子能谱仪(XPS)和飞行时间二次离子质谱仪(TOF-SIMS)对PAMEMA聚合物刷微图案的化学组成及分布进行表征，表明叠氮基团在聚合物刷图案化表面的区域选择性分布；以叠氮基团为反应位点，通过点击化学反应实现PAMEMA聚合物刷微图案表面的生物素化，借助荧光标记的链霉亲和素染色实验表征生物素在微图案表面的分布情况；以具有厚度变化的PAMEMA聚合物刷微图案为模板制备生物素表面，结果表明通过控制聚合物刷的厚度可以对微图案表面固定生物素分子的空间密度进行调控以实现具有复杂结构的生物素化梯度表面的成功制备.

Abstract

The biotin-avidin reaction system has shown remarkable advantages in immunoassays and biomedical fields due to its high specificity

excellent sensitivity and stability. The ability to generate biotinylated surface with gradient features would further promote the potential application of the biotin-avidin system in various fields ranging from high-throughput analysis to multifunctional biological surface. Here

based on the micropatterned polymer brushes functionalized with azido groups

a promising method was presented for fabricating the biotinylated gradient surfaces. Micropatterned poly(2-‍(2-azido-2-methyl-1-oxopropoxy) ethyl methacrylate) (PAMEMA) brushes bearing azido groups were fabricated through employing a digital mirror device (DMD)-based light modulation technique to regulate a surface-initiated photoinduced atom transfer radical polymerization (Photo-ATRP) process. X-ray photoelectron spectroscopy (XPS) and time of flight-secondary ion mass spectrometry (TOF-SIMS) measurements were performed to characterize the surface elemental composition of silicon substrates micropatterned with PAMEMA brushes

whose results indicate the spatially selective distribution of azido groups. The terminal azido groups of PAMEMA brushes could provide reactive sites for covalent immobilization of DBCO-PEG4-biotin through the highly specific orthogonal "click" reaction

leading to the biotinylation of PAMEMA brushes micropatterned surface. The streptavidin-fluorophore conjugate binding experiment was performed to characterize the distribution of biotins on the patterned surface. The fluorescence characterizations demonstrated the functionality of the micropatterned PAMEMA brushes with various thicknesses to regulate the spatial density of immobilized biotins

which resulted in the successful creation of biotinylated gradient surfaces with various complicated structures. The research extends the application of polymer brush micropattern in functional gradient surfaces preparation

and provides fundamental technical support for expanding biotinylated gradient surfaces applications in high-throughput detection chips and combinatorial chemistry.

关键词

聚(2-(2-叠氮-2-甲基丙氧基)甲基丙烯酸乙酯)刷微图案叠氮基团点击反应生物素化梯度表面

Keywords

Poly(2-(2-azido-2-methyl-1-oxopropoxy) ethyl methacrylate) brush micropatternAzido groupClick reactionBiotinylated gradient surface

references

Narupai B, Page Z A, Treat N J, McGrath A J, Pester C W, Discekici E H, Dolinski N D, Meyers G F, Read de Alaniz J, Hawker C J. Angew Chem Int Ed, 2018, 57(41): 13433-13438. doi:10.1002/anie.201805534http://dx.doi.org/10.1002/anie.201805534

Fors B P, Poelma J E, Menyo M S, Robb M J, Spokoyny D M, Kramer J W, Waite J H, Hawker C J. J Am Chem Soc, 2013, 135(38): 14106-14109. doi:10.1021/ja408467bhttp://dx.doi.org/10.1021/ja408467b

Bhat R R, Chaney B N, Rowley J, Liebmann-Vinson A, Genzer J. Adv Mater, 2005, 17: 2802-2807. doi:10.1002/adma.200500858http://dx.doi.org/10.1002/adma.200500858

Hu Yuanyuan(胡园园), Zhang Chaocan(张超灿), Chen Yanjun(陈艳军), Hu Liang(胡亮), Fan Yanan(范亚男). Acta Polymerica Sinica(高分子学报), 2011, (8): 838-844. doi:10.3724/sp.j.1105.2011.11099http://dx.doi.org/10.3724/sp.j.1105.2011.11099

Wu S, Du W, Duan Y, Zhang D, Liu Y, Wu B, Zou X, Ouyang H, Gao C. Acta Biomater, 2018, 75: 75-92. doi:10.1016/j.actbio.2018.05.046http://dx.doi.org/10.1016/j.actbio.2018.05.046

Masuda T, Akimoto A M, Nagase K, Okano T, Yoshida R. Sci Adv, 2016, 2: 1600902. doi:10.1126/sciadv.1600902http://dx.doi.org/10.1126/sciadv.1600902

Zhou K, Li Y, Zhang L, Jin L, Yuan F, Tan J, Yuan G, Pei J. Bioact Mater, 2021, 6(1): 262-272. doi:10.1016/j.bioactmat.2020.08.004http://dx.doi.org/10.1016/j.bioactmat.2020.08.004

Banuprasad T N, Vinay T V, Subash C K, Varghese S, George S D, Varanakkottu S N. ACS Appl Mater Interfaces, 2017, 9(33): 28046-28054. doi:10.1021/acsami.7b07451http://dx.doi.org/10.1021/acsami.7b07451

Carbonell C, Valles D, Wong A M, Carlini A S, Touve M A, Korpanty J, Gianneschi N C, Braunschweig A B. Nat Commun, 2020, 11(1): 1244. doi:10.1038/s41467-020-14990-xhttp://dx.doi.org/10.1038/s41467-020-14990-x

Rodenstein M, Zürcher S, Tosatti S G P, Spencer N D. Langmuir, 2010, 26(21): 16211-16220. doi:10.1021/la100805zhttp://dx.doi.org/10.1021/la100805z

Coad B R, Bilgic T, Klok H A. Langmuir, 2014, 30(28): 8357-8365. doi:10.1021/la501380mhttp://dx.doi.org/10.1021/la501380m

Yang Q, Wu T, Wang L, Wang Y, Chen Y, Chen L, Lou D, Cheng J, Liu D. Mater Chem Phys, 2021, 270: 124749. doi:10.1016/j.matchemphys.2021.124749http://dx.doi.org/10.1016/j.matchemphys.2021.124749

Zhou X C, Liu X Q, Xie Z, Zheng Z J. Nanoscale, 2011, 3(12): 4929. doi:10.1039/c1nr11238dhttp://dx.doi.org/10.1039/c1nr11238d

Xie Z, Gan T, Fang L, Zhou X. Soft Matter, 2020, 16(38): 8736-8759. doi:10.1039/d0sm01043jhttp://dx.doi.org/10.1039/d0sm01043j

Xue P, Liu W, Gu Z, Chen X, Nan J, Zhang J, Sun H, Cui Z, Yang B. ACS Appl Mater Interfaces, 2018, 11(1): 1595-1603. doi:10.1021/acsami.8b16547http://dx.doi.org/10.1021/acsami.8b16547

Wang C G, Yong H W, Goto A. ACS Appl Mater Interfaces, 2019, 11(15): 14478-14484. doi:10.1021/acsami.9b03570http://dx.doi.org/10.1021/acsami.9b03570

Bog U, de Los Santos Pereira A, Mueller S L, Havenridge S, Parrillo V, Bruns M, Holmes A E, Rodriguez-Emmenegger C, Fuchs H, Hirtz M. ACS Appl Mater Interfaces, 2017, 9(13): 12109-12117. doi:10.1021/acsami.7b01184http://dx.doi.org/10.1021/acsami.7b01184

Liu L, Tian X, Ma Y, Duan Y, Zhao X, Pan G. Angew Chem Int Ed, 2018, 57(26): 7878-7882. doi:10.1002/anie.201804802http://dx.doi.org/10.1002/anie.201804802

Xie Z, Chen C, Zhou X, Gao T, Liu D, Miao Q, Zheng Z. ACS Appl Mater Interfaces, 2014, 6(15): 11955-11964. doi:10.1021/am405555ehttp://dx.doi.org/10.1021/am405555e

Lu Z, Zhang Y, Wang Y, Tan G H, Huang F Y, Cao R, He N, Zhang L. J Control Release, 2021, 332: 245-259. doi:10.1016/j.jconrel.2021.02.029http://dx.doi.org/10.1016/j.jconrel.2021.02.029

Ren D, Xia Y, Wang J, You Z. Sensor Actuat B-Chem, 2013, 188: 340-346. doi:10.1016/j.snb.2013.07.037http://dx.doi.org/10.1016/j.snb.2013.07.037

Chen G, Guo P, Wang Y, Wang L, Shu H, Li Y, Jing W, Chang C, Fu Q. Talanta, 2019, 198: 55-62. doi:10.1016/j.talanta.2019.02.020http://dx.doi.org/10.1016/j.talanta.2019.02.020

Chen H A, Ma Y H, Hsu T Y, Chen J P. Int J Mol Sci, 2020, 21(8): 2690. doi:10.3390/ijms21082690http://dx.doi.org/10.3390/ijms21082690

Liu X, Zheng H N, Ma Y Z, Yan Q, Xiao S J. J Colloid Interf Sci, 2011, 358(1): 116-122. doi:10.1016/j.jcis.2011.03.013http://dx.doi.org/10.1016/j.jcis.2011.03.013

Zhao H L, Sha J, Wu T, Chen T, Chen X, Ji H, Wang Y, Zhu H, Xie L, Ma Y. Appl Surf Sci, 2020, 529: 147056. doi:10.1016/j.apsusc.2020.147056http://dx.doi.org/10.1016/j.apsusc.2020.147056

Parrillo V, de Los Santos Pereira A, Riedel T, Rodriguez-Emmenegger C. Anal Chim Acta, 2017, 971: 78-87. doi:10.1016/j.aca.2017.03.007http://dx.doi.org/10.1016/j.aca.2017.03.007

Views

228

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Synthesis and Self-assembly Behavior of Porphyrin-based Polypeptides

SYNTHESIS OF HBPS-PEO MULTI-ARM STAR POLYMER ELECTROLYTES AND THEIR IONIC CONDUCTIVITY

Related Author

Jia-ping Lin

Chun-hua Cai

Wei-an Zhang

Jia-hao Zheng

Yin-chao Wang

He-fei Chang

Yuan Lin

Hua-yi Li

Related Institution

School of Materials Science and Engineering, East China University of Science and Technology

University of Chinese Academy of Sciences

Beijing National Laboratory for Molecular Science, Key Laboratory of Engineering Plastics, Institute of Chemistry, Chinese Academy of Sciences

Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190

Postal code：100190
Tel：010-62588927 Email：gfzxb@iccas.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05002797号-8 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰