Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment

Hai-tao Yuan; Qi Shen; Wen Yu; Yue Zeng; Hao-tian Bai; Yi-ming Huang; Feng-ting Lv; Shu Wang

doi:10.11777/j.issn1000-3304.2022.22160

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Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment

Research Article | 更新时间：2024-10-08

- Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment
- ACTA POLYMERICA SINICA Vol. 53, Issue 10, Pages: 1251-1260(2022)
- 作者机构：
  
  中国科学院化学研究所有机固体重点实验室北京分子科学国家研究中心北京 100190
- 作者简介：
  
  Shu Wang, E-mail: wangshu@iccas.ac.cn
  Shu Wang, E-mail: wangshu@iccas.ac.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2022.22160
  CLC：
- Published：20 October 2022，
  
  Published Online：11 August 2022，
  
  Received：30 April 2022，
  
  Accepted：23 May 2022
- 稿件说明：
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袁海涛,申奇,于汶等.基于导电高分子-叶绿体杂化材料活性氧增强策略的肿瘤治疗[J].高分子学报,2022,53(10):1251-1260.

Yuan Hai-tao,Shen Qi,Yu Wen,et al.Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment[J].ACTA POLYMERICA SINICA,2022,53(10):1251-1260.
袁海涛,申奇,于汶等.基于导电高分子-叶绿体杂化材料活性氧增强策略的肿瘤治疗[J].高分子学报,2022,53(10):1251-1260. DOI： 10.11777/j.issn1000-3304.2022.22160.

Yuan Hai-tao,Shen Qi,Yu Wen,et al.Conductive Polymer-Chloroplast Living Material with Enhanced ROS Generation for Tumor Treatment[J].ACTA POLYMERICA SINICA,2022,53(10):1251-1260. DOI： 10.11777/j.issn1000-3304.2022.22160.

摘要

构建了一种基于共轭聚合物/叶绿体生物杂化材料的肿瘤治疗新方法. 共轭聚合物侧链带正电荷，能够与表面带负电荷的叶绿体通过静电作用结合，从而构造共轭聚合物/叶绿体生物杂化材料. 作为非生物成分，共轭聚合物PBF (poly(bor

on-dipyrromethene-

-fluorene))具有优异的光捕获和光转换能力，不仅提高了叶绿体对太阳光的利用效率，加速叶绿体内光反应中心的电子传递速率；而且进一步介导了光合作用中活性氧(ROS)的定向增强，实现肿瘤细胞的高效清除. 基于共轭聚合物/叶绿体生物杂化材料的肿瘤治疗方法为肿瘤治疗提供了一种新思路.

Abstract

A new antitumor treatment based on conjugated polymer/chloroplast biological hybrid material was proposed in this study

in which the conjugated polymer PBF (poly(boron-dipyrromethene-

-fluorene)) with a positive side chain charge could be electrostatically combined with negatively charged chloroplast. The successful combination of conjugated polymer PBF and chloroplast was demonstrated by the UV-Vis absorption spectra

the zeta potentials

and isothermal titration microcalorimetry (ITC) in sequence. In addition

the confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were utilized to visualize the structure of the PBF/chloroplast biological hybrid. The CLSM results demonstrated that the conjugated polymer PBF was closely surrounded by the chloroplast. The SEM image showed the PBF/chloroplast biological hybrid material was much rougher than that of the pristine chloroplast on account of the coating behavior of

conjugated polymer PBF. As an abiotic component

the conjugated polymer PBF possessed excellent light-harvesting and light conversion capability

which not only improved the utilization efficiency of sunlight in chloroplasts but also accelerated the electron transfer rate of the light reaction center in chloroplasts. The estimation was studied by the NADPH level of the live chloroplast. Under white light illumination

the NADPH in PBF/chloroplast was significantly increased compared to that of the pristine chloroplast. This increase in NADPH indicates that the photoinduced electron transfer by conjugated polymer PBF could improve the efficiency of NADPH-related intracellular reactions. The acceleration of the photosynthetic rate was also investigated through detecting the reduction rate of 2

6-dichlorophenolindophenol (DCPIP)

an artificial electron acceptor

which would capture the transported electrons of the chloroplast during the light reaction. Moreover

it further mediated the directional enhancement of reactive oxygen species (ROS) in photosynthesis to realize the efficient clearance of tumor cells. Overall

this work provided another new biological strategy based on conjugated polymer/chloroplast hybrid material for the development of treatments for malignant tumors.

关键词

共轭聚合物叶绿体光合作用活性氧增强肿瘤治疗

Keywords

Conjugated polymerChloroplast hybridPhotosynthesisReactive oxygen enhancementCancer therapy

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