Multi-functional Hydrogel Constructed by the Supramolecular Assembly between Ultralong DNA Chains and Tea Polyphenols

Jian-pu Tang; Zhen Cui; Jun-han Ou; Chi Yao; Da-yong Yang

doi:10.11777/j.issn1000-3304.2022.22173

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Multi-functional Hydrogel Constructed by the Supramolecular Assembly between Ultralong DNA Chains and Tea Polyphenols

Research Article | 更新时间：2024-10-08

- Multi-functional Hydrogel Constructed by the Supramolecular Assembly between Ultralong DNA Chains and Tea Polyphenols
- ACTA POLYMERICA SINICA Vol. 53, Issue 10, Pages: 1261-1270(2022)
- 作者机构：
  
  天津大学化工学院教育部合成生物学前沿科学中心系统生物工程教育部重点实验室天津 300350
- 作者简介：
  
  Chi Yao, E-mail: chi.yao@tju.edu.cn
  Da-yong Yang, E-mail: dayong.yang@tju.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2022.22173
  CLC：
- Published：20 October 2022，
  
  Published Online：11 August 2022，
  
  Received：07 May 2022，
  
  Accepted：28 June 2022
- 稿件说明：
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唐建普,崔臻,欧俊含等.超长单链DNA与茶多酚超分子组装构筑多功能水凝胶[J].高分子学报,2022,53(10):1261-1270.

Tang Jian-pu,Cui Zhen,Ou Jun-han,et al.Multi-functional Hydrogel Constructed by the Supramolecular Assembly between Ultralong DNA Chains and Tea Polyphenols[J].ACTA POLYMERICA SINICA,2022,53(10):1261-1270.
唐建普,崔臻,欧俊含等.超长单链DNA与茶多酚超分子组装构筑多功能水凝胶[J].高分子学报,2022,53(10):1261-1270. DOI： 10.11777/j.issn1000-3304.2022.22173.

Tang Jian-pu,Cui Zhen,Ou Jun-han,et al.Multi-functional Hydrogel Constructed by the Supramolecular Assembly between Ultralong DNA Chains and Tea Polyphenols[J].ACTA POLYMERICA SINICA,2022,53(10):1261-1270. DOI： 10.11777/j.issn1000-3304.2022.22173.

摘要

为开发新的DNA组装模式、整合杂化功能模块、丰富DNA水凝胶的合成方法和功能，利用双滚环扩增策略合成了超长DNA链，通过茶多酚(TP)与DNA链间的超分子组装，构建了TP-DNA杂化水凝胶. 优化了水凝胶组分，通过扫描电子显微镜、流变测试等对水凝胶进行表征，以此研究TP的引入对DNA水凝胶性能的影响. 研究结果表明，TP的引入不仅可以调节DNA水凝胶受核酸酶降解的速率，还赋予水凝胶抑菌、促进伤口愈合的特性，在大鼠伤口模型中显著地促进伤口愈合.

Abstract

As a biological polymeric material

deoxyribonucleic acid (DNA) possesses unique sequence programmability

structural predictability and functional designability. Recently

DNA hydrogel has been widely used in bioimaging

drug delivery

the separation of biological particle and the intervention of cell behavior. However

the materials with DNA as the sole module face the challenges of structural singleness and functional limitations. To explore more synthesis methods and functions of DNA hydrogel

researchers have introduced hybrid functional modules to develop new molecular assembly strategies. As natural polyphenols

tea polyphenols (TP) have been proven to have good antioxidant

antibacterial

antiviral and anti-tumor activities

which make TP potential for various biological applications. Previous studies have shown that stable hydrogen bonds can be formed between the phosphate groups of DNA and the phenolic hydroxyl groups of polyphenols. Herein

a TP-DNA hydrogel based on ultralong DNA chains was synthesized by double rolling circle amplification strategy and the assembly of TP with DNA. The mass ratio of TP to DNA in TP-DNA hybrid hydrogels was optimized. The results of scanning electron microscopy and rheological test showed that TP-DNA hydrogel possessed porous structure and shear thinning property. On the other hand

the experiments of nuclease resistance

biocompatibility

antibacterial activity and wound healing evaluation were performed to explore the effect of TP on the properties of DNA hydrogels. The results showed that TP not only delayed the degradation rate of DNA hydrogel under nuclease

but also endowed the hydrogel with good antibacterial activity. In a rat wound healing model

the TP-DNA hydrogel was proven to significantly promote wound healing.

关键词

DNA水凝胶茶多酚抗菌抗降解

Keywords

DNA hydrogelTea polyphenolsAntibacterialAntidegradation

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