Preparation and Performance Study of Lipoic Acid-enhanced Double-network Silk Fibroin Hydrogel Dressing

Lu-hang Fang; Xiao-yang Liang; Zi-yi Shen; Chen Xu; Yang Li; Fu-Jian Xu

doi:10.11777/j.issn1000-3304.2026.25311

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Preparation and Performance Study of Lipoic Acid-enhanced Double-network Silk Fibroin Hydrogel Dressing

更新时间：2026-03-25

- Preparation and Performance Study of Lipoic Acid-enhanced Double-network Silk Fibroin Hydrogel Dressing
- Acta Polymerica Sinica Pages: 1-12(2026)
- 作者机构：
  
  北京化工大学化学资源工程国家重点实验室天然高分子基医用材料教育部重点实验室（北京化工大学）生物医用材料北京市实验室北京 100029
- 作者简介：
  
  Yang Li, E-mail: ly@mail.buct.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2026.25311
  CLC：
- CSTR：32057.14.GFZXB.2026.7556
- Received：22 December 2025，
  
  Accepted：09 February 2026，
  
  Online First：25 March 2026，
- 稿件说明：
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方路航, 梁晓炀, 申子怡, 许晨, 李杨, 徐福建. 硫辛酸增强的双网络丝素蛋白水凝胶敷料的制备及性能研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.25311.

Fang, L. H.; Liang, X. Y.; Shen, Z. Y.; Xu, C.; Li, Y.; Xu, F. J. Preparation and performance study of lipoic acid-enhanced double-network silk fibroin hydrogel dressing. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.25311.
方路航, 梁晓炀, 申子怡, 许晨, 李杨, 徐福建. 硫辛酸增强的双网络丝素蛋白水凝胶敷料的制备及性能研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2026.25311. DOI： CSTR： 32057.14.GFZXB.2026.7556.

Fang, L. H.; Liang, X. Y.; Shen, Z. Y.; Xu, C.; Li, Y.; Xu, F. J. Preparation and performance study of lipoic acid-enhanced double-network silk fibroin hydrogel dressing. Acta Polymerica Sinica (in Chinese), doi: 10.11777/j.issn1000-3304.2026.25311. DOI： CSTR： 32057.14.GFZXB.2026.7556.

摘要

单一网络的丝素蛋白水凝胶缺少力学强度及其他生物活性. 本工作通过光聚合制备甲基丙烯酰化改性的丝素蛋白(SFMA)水凝胶，再与硫辛酸(LA)、精氨酸(Arg)共混一步浸泡法热聚合制备双网络复合水凝胶(SFLA). 首先通过扫描电子显微镜、流变学等方法对不同浓度SFMA的微观结构和力学性能进行表征，随后通过成胶筛选出LA和Arg的适宜浓度，从而制备出SFLA水凝胶并通过紫外测定水凝胶的药物释放情况，最后对水凝胶的生物安全性、抗氧化性和抗菌能力进行测定. 结果表明在丝素蛋白单网络水凝胶中引入LA交联的次级网络可以增强水凝胶的力学强度和黏附性能，同时通过LA和Arg的协同作用赋予了水凝胶优异的抗氧化及抗菌能力.

Abstract

Silk fibroin hydrogels with a single network lack mechanical strength and other bioactivities. To address this issue

methacrylated silk fibroin (SFMA) hydrogels were first prepared

via

photopolymerization. Subsequently

double-network composite hydrogels (SFLA) were fabricated through one-step immersion thermal polymerization by blending SFMA with lipoic acid (LA) and arginine (Arg). First

the microstructure and mechanical properties of SFMA hydrogels with different concentrations were determined using scanning electron microscopy and rheology. The appropriate concentrations of LA and Arg were screened through gelation tests

and SFLA hydrogels were prepared. The drug release behavior of the hydrogels was measured using ultraviolet (UV) spectroscopy. Finally

the biosafety

antioxidant activity

and antibacterial properties of the hydrogels were determined. The results showed that introducing a secondary network cross-linked by LA into the silk fibroin single-network hydrogels enhanced the mechanical strength and adhesion performance of the hydrogels. The synergistic effect of LA and Arg endowed the hydrogels with excellent antioxidant and antibacterial capabilities.

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