Poria cocos mycelia were cultivated from artificial strain No 5·78 in medium containing bran at 25℃ for eighteen days．Seven polysaccharides coded as ab-PCMl

ab-PCM2-I

ab-PCM2-II

ab—PCM3-I

ab-PCM3-II

ab-PCM4-I and ab-PCM4-II were isolated from the poria cocos mycelia by using 9 g/L NaCI aqueous solution

hot water

0.5 mol／L NaOH aqueous solution and 88％ formic acid

respectively

and an exo-polysaccharide coded as ab-PCMO was isolated from the medium. The components of the polysaccharides were analyzed by using infrared spectroscopy(IR)

gas chromatography (GC) and 13C-NMR．The results indicated that the polysaccharides from ab-PCMO to ab-PCM3-I consist ofα-(1→3)-D-glucose

α-D-mannose

β-D-galactose

and N-acetyl glucosamine

and with the isolation progress the glucose content in the polysaccharides increased

namely from heteropolysaccharide toα-glucan．The ab-PCM3-II

ab-PCM4-I and ab-PCM4-II mainly consist of β-(1→3)-D-glucan．Interestingly

α-glucan and β-glucan coexist in the alkali extracted solution of the mycelia．The intrinsic viscosities [η] and weight average molecular weights Mw were determined by viscometry and light scattering．The values of Mw for samples of ab-PCM0

ab-PCM1

ab-PCM2-I

ab-PCM2-II

ab-PCM3-I and ab-PCM4-II are 7.7×104

5.2×104

5.0×104

24.3×lO4

65.2 x 104 and 25.5×lO4

respectively．