STUDY ON THE FABRICATION AND THE ACTIVITY OF HORSERADISH PEROXIDASE BIOFILMS SELF-ASSEMBLED BY PREMIXING METHOD

JIANG Xiuming; CHENG Zhichun; YANG Shaoming; LIN Xianfu

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STUDY ON THE FABRICATION AND THE ACTIVITY OF HORSERADISH PEROXIDASE BIOFILMS SELF-ASSEMBLED BY PREMIXING METHOD

Research Article | 更新时间：2021-03-19

- STUDY ON THE FABRICATION AND THE ACTIVITY OF HORSERADISH PEROXIDASE BIOFILMS SELF-ASSEMBLED BY PREMIXING METHOD
- Acta Polymerica Sinica Issue 2, Pages: 181-185(2005)
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- Published：20 April 2005，
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JIANG Xiuming, CHENG Zhichun, YANG Shaoming, LIN Xianfu. STUDY ON THE FABRICATION AND THE ACTIVITY OF HORSERADISH PEROXIDASE BIOFILMS SELF-ASSEMBLED BY PREMIXING METHOD. [J]. Acta Polymerica Sinica (2):181-185(2005)
DOI：

JIANG Xiuming, CHENG Zhichun, YANG Shaoming, LIN Xianfu. STUDY ON THE FABRICATION AND THE ACTIVITY OF HORSERADISH PEROXIDASE BIOFILMS SELF-ASSEMBLED BY PREMIXING METHOD. [J]. Acta Polymerica Sinica (2):181-185(2005) DOI：

摘要

以阳离子化的辣根过氧化物酶 (HRP)和阴离子聚苯乙烯磺酸钠 (PSS)的预混合溶液

与阳离子聚电解质聚二甲基二烯丙基氯化铵 (PDDA)通过逐层组装

在阴离子化聚对苯二酸乙二酯 (PET)表面构建了多层生物活性膜 .用紫外可见光谱仪 (UV-Vis)和原子力显微镜 (AFM)研究了交替自组装膜的结构和表面形膜

并测定了自组装膜的生物催化活性 .结果表明

预混合溶液中的PSS与HRP一起沉积在PDDA膜层上组装成 (PSS+HRP)膜层

且每层中PSS和HRP的比例一致;(PSS+HRP)膜层呈条状分布

膜表面较为平整;多层膜中的HRP催化H2O2与 4-氨基安替比林的显色反应的表观米氏常数为 9.7×10-5mol·L-1 (相对于H2O2 底物 )

较溶液中 (1.52×10-4mol·L-1)的小.

Abstract

The premixing self-assembly solution of horseradish peroxide with poly(sodium 4-styrenesulfonate)(RRP +PSS) is made by blending HRP and polyelectrolyte PSS

and the active[(HRP+PSS)/PDDA]。enzyme muhilayer biofilm is fabricated by altemate deposition of (HRP+PSS) and PDDA on the surface of negative ionized PET．The topography of films

the stepwise growth of layers and the activity of HRP in the film are measured with UV-Vis and AFM．HRP and PSS are assembled on the PDDA layer together from the premixing solution of (HRP+PSS)

and the ratio of HRP to PSS in every layer of(HRP+PSS) is the same．The banded stmcture of HRP under the PSS is observed on the PDDA layer．The Michaelis Constant of the reaction of H2O2 and 4-amino-antipyrine(4-AAP) catalyzed by HRP in biofilm is 9.7×10-5mol·L-1 and is lowet than that of the reaction catalyzed by HRP in solution (1.52×10-4 mol·L-1)．The affinity between H2O2

and HRP in biofflms is rather strong．

关键词

逐层自组装辣根过氧化物酶聚电解质原子力显微镜紫外-可见光谱

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College of Science, Dalian Ocean University

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