A three-dimensional (3D) ordered structure was achieved by self-organization of monodisperse polystyrene latex about 250 nm in diameter after being ambient dried. The stacked morphology was investigated by scanning electron microscopy(SEM) observation．It is shown that the face-centered cubic(FCC) phase dominantly exists．This finding presents a potential template for preparing an inverse FCC structure as a photonic crystal of full band gap if the index contrast is higher than 2.8．