Functiona poly(lactic acid) microspheres with cole-shell structures have been prepared by using the PEO-PPO-PEO(Pluronics F68) or the ligand-tethered PEO-PPO-PEO derivatives as the surface stabilizer and poly-(lactic acid) as the core material．Confocal Laser Scanning Microscopy (CLSM) investigation indicated that the amphiphillic triblock copolymer PEO-PPO-PEO or its ligand-tethered derivatives existed on the shell of microspheres. The ligand-tethered microspheres were then embedded in cell-resistant PEO-PPO-PEO films to create cell-interactive surfaces with discrete clustered-ligand domains．The osteoblast compatibility test showed that the amino acid or peptide RGD existed on the surface of microspheres promoted osteoblast attachment and growth．This simple surface treatment method may have potentials for tissue engineering and other biomedical applications．