Uniformly imprinted polymer microspheres were prepared by precipitation polymerization in chloroform．MCP(monocrotophos)

MAA(methacrylie acid)

EGDMA(ethylene glycol dimethacrylate)and AIBN (azobisisobutyronitrile)were used as template

functional monomer

cross-linker and initiator

respectively．UV and IR spectra were used to demonstrate the mechanism of the interaction between MCP and MAA．It was found that one MCP was entrapped by three MAA molecules in chloroform．Langmuir model was used to fit the adsorption data

the results showed that the MCP imprinted polymer microspheros showed specific binding sites to the MCP．The data obtained in equilibrium adsorption experiments were processed by Seatehard analysis

the dissociation contents (KDl)and the apparent maximum binding capacity(Bmax1)were KDl=0．062mmol／L

Bmaxl=3．26μmol／g for high affinity binding sites and KD2=3．03 mmol／L

Bmax2=65．70μmol／g for low affinity binding sites

respectively．The MCP imprinted polymers have showed hisher affinity to MCP than to the other organophosphorus pesticide used．So the imprinted polymers mierospheres can be used for trace pesticide residue separation

enrichment and analysis．