THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN

ZHANG Jing; ZHANG Zhengpu; HE Bingkun

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THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN

Research Bulletin | 更新时间：2021-03-19

- THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN
- Acta Polymerica Sinica Issue 4, Pages: 640-644(2006)
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- Published：20 July 2006，
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ZHANG Jing, ZHANG Zhengpu, HE Bingkun. THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN. [J]. Acta Polymerica Sinica (4):640-644(2006)
DOI：

ZHANG Jing, ZHANG Zhengpu, HE Bingkun. THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN. [J]. Acta Polymerica Sinica (4):640-644(2006) DOI：

摘要

Abstract

A series of aminated chitosan microspheres were prepared from chitosan

and then an affinity dye—ligand

Cibacron Blue F3GA

was covalently coupled．The maximum attachment of cibacron blue F3GA was 494 μmol／g．Albumin adsorption onto cibacron blue F3GA—attached chitosan microspheres was investigated．The human serum albumin(HSA)equilibrium adsorption capacity WaS 79．8 mg／s(wet beads)．The adsorption equilibrium isotherms appeared to follow a typical Langmuir isotherm．The hisheet desorption ratio(100％)was achieved by using an eluent of 1．0 mol／L NaSCN(pH 8．0)．The cibacron blue F3GA-attached chitosan microspheres could be reused without significant decreases in the adsorption capacities．

关键词

壳聚糖Cibacron Blue F3GA染料亲和吸附剂HAS

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