This paper reports a technique to fabricate the stationary phase for high—speed protein flowthrough chromatography．Using glycerol aqueous solution(20 wt％)and n-heptane／toluene organic solvent as the porogenic agents

a novel type of bipomus matrix(BiPB)has been prepared by ultraviolet photo—polymerizing monomers (glycidyl methacrylate and ethylene glycol dimethacrylate)by a(W／O)／W emulsification technique．It was found that there were two types of pores(micropores and superpores)in BiPB

but there was only micropores in the micropomus beads(MiPB)created by O／W emulsification．The pores of BiPB were in the range of 10—100 nm and 1000～7300 nm

whereas those of MiPB were mainly 10～100 nm in size．The specific surface alea

hydrated density and particle size of BiPB were 41．9m2/g

1．08 g/mL and 42．8 μm

respectively

similar to those of MiPB(52．1m2／g

1．11 g/mL and 42．4 μm)．The static adsorption capacity of BiPB for bovine serum albumin(BSA)was also similar to that of MiPB．At a low flow rate(5cm／min)

the dynamic adsorption eapacity(DAC)of BiPB for BSA was close to that of MiPB．At high flow rate(40 cm／min)

however

the DAC of BiPB Was about three times as high as that of MiPB．The results indicate that BiPB columns were promising for high—speed protein chromatography．