The synthetic thermo-responsive polymer azidoaniline-coupled poly( N-isopropylacrylamide/acrylic acid)(AzPhPIA) was photo-immobilized on Polystyrene Culture Plates. On the plates

STO cells were cultured for two hours. Under the low critical solution temperature (LCST 21.5℃)

detachment experiment showed the cells which were cultured on the thermo-responsive polymer could not be detached completely as the serum-free cell culture. For investigating the effects of serum

we chose two serum protein (albumin and fibronectin) for cell attachment and detachment. The results showed that fibronectin was coated all the surface of plate

but albumin was pattern-coated on the plate and liked to coated on the surface of polystyrene. It was fibronectin which increased cell attachment so that cells soulld not be detached

and was due to hydrophilic of thermo-responsive polymer AzPhPIA not the attachment influence of albumin that cells were detached under the LCST.Surface analysis demonstrated that coated protein would not affect the wettability of AzPhPIA.