ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA

DING Qiong; ZHANG Lina; CHEUNG Peter Chi Keung

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ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA

Research Article | 更新时间：2021-03-19

- ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA
- Acta Polymerica Sinica Issue 2, Pages: 224-227(2000)
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- Published：20 April 2000，
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DING Qiong, ZHANG Lina, CHEUNG Peter Chi Keung. ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA. [J]. Acta Polymerica Sinica (2):224-227(2000)
DOI：

DING Qiong, ZHANG Lina, CHEUNG Peter Chi Keung. ISOLATION AND STRUCTURAL ANALYSIS OF POLYSACCHARIDES FROM PORIA COCOS MYCELIA. [J]. Acta Polymerica Sinica (2):224-227(2000) DOI：

摘要

由茯苓菌种在培养基中于 2 5℃下培养一周得茯苓菌丝体 .分别用 0.9%NaCl水溶液、热水、0.5mol/LNaOH和 88%甲酸从该茯苓菌丝体中提取出四种多糖

编号为PCM1

PCM2

PCM3和PCM4 .用红外光谱 (IR)

高效液相色谱 (HPLC)

气相色谱 (GC)及13C-核磁共振 (13C-NMR)等方法分析了它们的组成和结构 .结果表明

PCM1

PCM2为酸性杂多糖由D 鼠李糖、D-木糖、D-甘露糖、D-半乳糖、D-葡萄糖及葡萄糖醛酸组成 .PCM3主要为线型 β-( 1→ 3 ) D-葡聚糖

其产率占茯苓菌丝体总量的 55.8% .PCM4由D-葡萄糖和葡萄糖醛酸组成 .茯苓菌丝体化学组成和结构基本同于茯苓菌核多糖

随提取过程进行葡萄糖含量逐渐增加的变化规律也相同 .

Abstract

The poria cocos mycelia samples were cultured in media at 25℃ for one week.Four polysaccharides coded as PCM1

PCM2

PCM3 and PCM4 were isolated from the poria cocos mycelia by using 0.9% NaCl aqueous solution

hot water

0.5mol/L NaOH aqueous solution and 88% formic acid

respectively.The components and structure of the polysaccharides were analyzed by infrared

gas chromatography

high performance liquid chromatography and 13C nuclear magnetic resonance.The results show that PCM1 and PCM2 consist of D-rhamnose

D-fucose

D-xylose

D-mannose

D-galactose

D-glucose and D-glucuronic acid

and the D-glucose content increases with the progress of isolation.PCM3 is mainly linear β-(1→3)-D-glucan and the major fraction of polysaccharides fron the poria cocos mycelia (yield 55.8%).PCM4 is composed of D-glucose with a few glucuronic acids.It is summarized that the coomponent and structure of the polysaccharides from poria cocos mycelia are similar to those from poria cocos sclerotium.

关键词

茯苓菌丝体多糖结构NMR

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