The a-amino acid functionalized asymmetric resins were prepared by the reaction of the aniliniuin resins with a-amino acids，or by the amination of chloromethylated styrene-divinylbenzene copolymers with a-amino acids in the presence of phase-transfer catalysts for chromatographic separation of racemic compound．The structures of the asrmmetric resins were confimed by infrared spectroscopic analysis．The factors affecting the properties of the asymmetric resins，such as the pro-perties of solvents，amount of catalysts，the temperature and time of the reaction etc.，were．investigated．The L-proline functionalized asymmetric resin was prepared by the two aforesaid procedures．The copper (II) complex of the L-proline functionalized asymmetric resin was utilized for ligand-exchange chromatography of the resolution of DL-proline，thus，we resolved the racemic mixture into the D-and L-proline．