In this paper

the conformational transition of silk fibroin in different parts of silkgland was determinated by Raman spectroscopy and LAXS. It was discovered that the β-sheet conformation of fibroin came into being gradually when the stress was increased. By stretching the fresh fibroin directly with Instron

their conformational transitions were studied by Raman spectroscopy. The denaturing process of silk fibroin under stress was revealed on micro-cosmic levels

and a relevant model was made.