Preparation and Performance Study of Hypercrosslinked Adsorbents with Functional Groups for Whole Blood Hemoperfusion

Ke Shao; Xin-yan Peng; Yun-hong Liu; Yue-lan Wei; Kai-wen Chen; Xiang Zhou

doi:10.11777/j.issn1000-3304.2025.25080

您当前的位置：

首页 >

文章列表页 >

Preparation and Performance Study of Hypercrosslinked Adsorbents with Functional Groups for Whole Blood Hemoperfusion

Research Article | 更新时间：2025-10-29

- Preparation and Performance Study of Hypercrosslinked Adsorbents with Functional Groups for Whole Blood Hemoperfusion
- Acta Polymerica Sinica Vol. 56, Issue 11, Pages: 2079-2094(2025)
- 作者机构：
  
  1.泉州师范学院化工与材料学院泉州 362000
  2.健帆生物科技集团股份有限公司珠海 519085
  3.广州职业技术大学珠宝学院广州 510640
- 作者简介：
  
  Xin-yan Peng, E-mail: pengxinyan@qztc.edu.cn
  Yun-hong Liu, E-mail: liuyunhong@qztc.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2025.25080
  CLC：
- CSTR：32057.14.GFZXB.2025.7445
- Received：25 March 2025，
  
  Accepted：27 June 2025，
  
  Published Online：03 September 2025，
  
  Published：20 November 2025
- 稿件说明：
移动端阅览
邵柯, 彭新艳, 刘云鸿, 韦跃兰, 陈凯文, 周翔. 全血灌流用含功能基团超交联吸附剂的制备与性能研究. 高分子学报, 2025, 56(11), 2079-2094

Shao, K.; Peng, X. Y.; Liu, Y. H.; Wei, Y. L.; Chen, K. W.; Zhou, X. Preparation and performance study of hypercrosslinked adsorbents with functional groups for whole blood hemoperfusion. Acta Polymerica Sinica, 2025, 56(11), 2079-2094
邵柯, 彭新艳, 刘云鸿, 韦跃兰, 陈凯文, 周翔. 全血灌流用含功能基团超交联吸附剂的制备与性能研究. 高分子学报, 2025, 56(11), 2079-2094 DOI： 10.11777/j.issn1000-3304.2025.25080. CSTR： 32057.14.GFZXB.2025.7445.

Shao, K.; Peng, X. Y.; Liu, Y. H.; Wei, Y. L.; Chen, K. W.; Zhou, X. Preparation and performance study of hypercrosslinked adsorbents with functional groups for whole blood hemoperfusion. Acta Polymerica Sinica, 2025, 56(11), 2079-2094 DOI： 10.11777/j.issn1000-3304.2025.25080. CSTR： 32057.14.GFZXB.2025.7445.

摘要

在临床尿毒症血液灌流治疗领域，超交联聚苯乙烯吸附剂已得到了广泛的应用，但仍面临着材料制备和改性过程繁琐复杂、以及对蛋白结合类毒素(PBUTs)吸附清除能力不足的问题. 为解决上述问题，本研究采用一步法外交联改性制备技术，利用带有特定功能基团的小分子外交联剂，如(二甲氨基)乙

醛缩二甲醇(DDA)和氨基乙醛缩二甲醇(DMEA)，对一次交联聚苯乙烯树脂PDVB进行同步后交联和改性，成功制备出功能化超交联聚苯乙烯吸附剂HCP-DMEA和HCP-DDA. 利用红外光谱、光电子能谱、扫描电镜及BET等技术手段，对超交联树脂的物理化学结构进行了系统表征. 研究结果表明，含功能基团小分子外交联剂可对一次交联树脂同时实现后交联反应和基团改性，成功在吸附剂结构中引入不同含氮基团；所制备的HCP-DMEA和HCP-DDA树脂同时具有较好的尿毒症蛋白结合类毒素(IS，pCS)和中大分子毒素(

2-MG，IL-6)吸附性能. 与商用HA130树脂相似，本研究制备的超交联树脂具有较好的血液相容性，有望在全血灌流、人工肾等领域广泛应用，为尿毒症患者提供更高效、更安全的治疗选择.

Abstract

In the field of clinical uremic blood perfusion therapy

hypercrosslinked polystyrene adsorbents have been widely applied. However

they still face challenges

including the complexity of material preparation and modification processes

as well as insufficient adsorption capabilities for protein-bound uremic toxins (PBUTs). To address the aforementioned issues

this study employs a one-step external crosslinking modification technique

utilizing small-molecule external crosslinkers with specific functional groups

such as (dimethylamino)acetaldehyde dimethyl acetal (DDA) and aminoacetaldehyde dimethyl acetal (DMEA)

to simultaneously perform post-crosslinking and modification on the pre-crosslinked polystyrene resin PDVB. This process successfu

lly produced functionalized hyper-crosslinked polystyrene adsorbents HCP-DMEA and HCP-DDA. The physicochemical structures of the hypercrosslinked resins were characterized and analyzed using techniques such as Fourier infrared spectroscopy (FTIR)

X-ray photoelectron spectroscopy (XPS)

scanning electron microscopy (SEM)

and Brunauer-Emmett-Teller (BET) surface area analysis. The research results indicated that small-molecule external crosslinkers containing functional groups could simultaneously achieve post-crosslinking reactions and group modifications on the pre-crosslinked resin

successfully introducing different nitrogen-containing groups into the adsorbent structure. The prepared HCP-DMEA and HCP-DDA resins exhibited excellent adsorption performance for both PBUTs (IS

pCS) and middle-to-large molecular toxins (

2-MG

IL-6). Similar to the commercial HA130 resin

these hypercrosslinked resins demonstrated good blood compatibility and holded promising potential for wide application in whole blood hemoperfusion

artificial kidneys

and related fields. It is expected to provide more efficient and safer treatment options for patients with uremia.

关键词

Keywords

references

Dou W. Y. ; Wang J. ; Yao Z. K. ; Xiao W. ; Huang M. ; Zhang L. A critical review of hemoperfusion adsorbents: materials, functionalization and matrix structure selection . Mater. Adv. , 2022 , 3 ( 2 ), 918 - 930 . doi: 10.1039/d1ma00892g http://dx.doi.org/10.1039/d1ma00892g

刘莎 , 刘文虎 . 血液灌流技术应用与进展 . 中国血液净化 , 2008 , 7 ( 11 ), 618 - 621 . doi: 10.3969/j.issn.1671-4091.2008.11.011 http://dx.doi.org/10.3969/j.issn.1671-4091.2008.11.011

Peng Y. T. ; Feng X. Y. ; Jiang J. M. ; Ren L. Controllable polyvinylpyrrolidone modified Polystyrene divinylbenzene for efficient adsorption of bilirubin and improvement of hemocompatibility . Eur. Polym. J. , 2022 , 170 , 111172 . doi: 10.1016/j.eurpolymj.2022.111172 http://dx.doi.org/10.1016/j.eurpolymj.2022.111172

Feng X. Y. ; Li J. C. ; Peng Y. T. ; Guo W. T. ; Liang L. J. ; Zhu L. L. ; Liu S. ; Ren L. Zwitterionic polymers as high-performance coatings for hemoperfusion adsorbents and their chemisorption of protein-bound toxins through computational simulations . Colloids Surf. A Physicochem. Eng. Aspects , 2024 , 680 , 132710 . doi: 10.1016/j.colsurfa.2023.132710 http://dx.doi.org/10.1016/j.colsurfa.2023.132710

Pavlenko D. ; Giasafaki D. ; Charalambopoulou G. ; van Geffen E. ; Gerritsen K. F. ; Steriotis T. ; Stamatialis D. Carbon adsorbents with dual porosity for efficient removal of uremic toxins and cytokines from human plasma . Sci. Rep. , 2017 , 7 ( 1 ), 14914 . doi: 10.1038/s41598-017-15116-y http://dx.doi.org/10.1038/s41598-017-15116-y

Liu Y. H. ; Peng X. Y. ; Hu Z. D. ; Yu M. G. ; Fu J. J. ; Huang Y. G. Fabrication of a novel nitrogen-containing porous carbon adsorbent for protein-bound uremic toxins removal . Mater. Sci. Eng. C , 2021 , 121 , 111879 . doi: 10.1016/j.msec.2021.111879 http://dx.doi.org/10.1016/j.msec.2021.111879

Gao X. T. ; Liu K. ; Liu P. ; Bai X. Y. ; Li A. X. ; Lyu Z. J. ; Li Q. H. Preparation and properties of cellulose acetate graft copolymer-coated adsorbent resin for hemoperfusion device . J. Appl. Polym. Sci. , 2023 , 140 ( 19 ), e 53895 . doi: 10.1002/app.53895 http://dx.doi.org/10.1002/app.53895

Wang Z. H. ; Cao Y. H. ; Gu T. X. ; Wang L. P. ; Chen Q. ; Wang J. M. ; Zhao C. S. Biomimetic porous MXene antibacterial adsorbents with enhanced toxins trapping ability for hemoperfusion . Small , 2024 , 20 ( 45 ), e 2403271 . doi: 10.1002/smll.202403271 http://dx.doi.org/10.1002/smll.202403271

Zhang M. Y. ; Li L. ; Lei L. C. ; Kang K. ; Xiao C. L. Effectively decontaminating protein-bound uremic toxins in human serum albumin using cationic metal-organic frameworks . ACS Appl. Mater. Interfaces , 2022 , 14 ( 50 ), 55354 - 55364 . doi: 10.1021/acsami.2c15864 http://dx.doi.org/10.1021/acsami.2c15864

Cuchiaro H. ; Thai J. ; Schaffner N. ; Tuttle R. R. ; Reynolds M. Exploring the parameter space of p -cresyl sulfate adsorption in metal-organic frameworks . ACS Appl. Mater. Interfaces , 2020 , 12 ( 20 ), 22572 - 22580 . doi: 10.1021/acsami.0c04203 http://dx.doi.org/10.1021/acsami.0c04203

Ronco C. The promise of adsorption for chronic dialysis patients . Blood Purif. , 2022 , 1 - 4 . doi: 10.1159/000525953 http://dx.doi.org/10.1159/000525953

Graf H. ; Gräfe C. ; Bruegel M. ; Happich F. L. ; Wustrow V. ; Wegener A. ; Wilfert W. ; Zoller M. ; Liebchen U. ; Paal M. ; Scharf C. Extracorporeal elimination of pro- and anti-inflammatory modulators by the cytokine adsorber CytoSorb ® in patients with hyperinflammation: a prospective study . Infect. Dis. Ther. , 2024 , 13 ( 9 ), 2089 - 2101 . doi: 10.1007/s40121-024-01028-8 http://dx.doi.org/10.1007/s40121-024-01028-8

Koler A. ; Pulko I. ; Krajnc P. Post polymerisation hypercrosslinking with emulsion templating for hierarchical and multi-level porous polymers . Acta Chim. Slov. , 2020 , 67 ( 2 ), 349 - 360 . doi: 10.17344/acsi.2020.5901 http://dx.doi.org/10.17344/acsi.2020.5901

Ahn J. H. ; Jang J. E. ; Oh C. G. ; Ihm S. K. ; Cortez J. ; Sherrington D. C. Rapid generation and control of microporosity, bimodal pore size distribution, and surface area in davankov-type hyper-cross-linked resins . Macromolecules , 2006 , 39 ( 2 ), 627 - 632 . doi: 10.1021/ma051152n http://dx.doi.org/10.1021/ma051152n

曾小伟 . 悬挂双键后交联吸附树脂的结构设计、极性修饰及其性能研究 . 南开大学博士学位论文 , 2011 .

Li B. Y. ; Gong R. N. ; Wang W. ; Huang X. ; Zhang W. ; Li H. M. ; Hu C. X. ; Tan B. E. A new strategy to microporous polymers: knitting rigid aromatic building blocks by external cross-linker . Macromolecules , 2011 , 44 ( 8 ), 2410 - 2414 . doi: 10.1021/ma200630s http://dx.doi.org/10.1021/ma200630s

Maya F. ; Svec F. A ne w approach to the preparation of large surface area poly(styrene-co-divinylbenzene) monoliths via knitting of loose chains using external crosslinkers and application of these monolithic columns for separation of small molecules . Polymer , 2014 , 55 ( 1 ), 340 - 346 . doi: 10.1016/j.polymer.2013.08.018 http://dx.doi.org/10.1016/j.polymer.2013.08.018

Ghafari M. ; Atkinson J. D. One-step hyper-cross-linking of porous styrenic polymers using dichloroalkane cross-linkers to maintain hydrophobicity . Polymer , 2017 , 116 , 278 - 286 . doi: 10.1016/j.polymer.2017.03.082 http://dx.doi.org/10.1016/j.polymer.2017.03.082

Liu Y. H. ; Peng X. Y. Multi-functional hypercrosslinked polystyrene as high-performance adsorbents for artificial liver blood purification . Front. Chem. , 2022 , 9 , 789814 . doi: 10.3389/fchem.2021.789814 http://dx.doi.org/10.3389/fchem.2021.789814

Qin T. T. ; Gao L. ; Zhao W. J. Application progress of hypercrosslinked porous organic polymers in cartridge-based solid phase extraction . Chin. J. Chromatogr. , 2023 , 41 ( 7 ), 554 - 561 . doi: 10.3724/sp.j.1123.2022.12003 http://dx.doi.org/10.3724/sp.j.1123.2022.12003

Chen L. Y. ; Liu Y. H. ; Peng X. Y. ; Wei Y. L. ; Shao K. Simultaneous hypercrosslinking and functionalization of porous polystyrene adsorbent for protein-bound uraemic toxins removal . React. Funct. Polym. , 2025 , 214 , 106265 . doi: 10.1016/j.reactfunctpolym.2025.106265 http://dx.doi.org/10.1016/j.reactfunctpolym.2025.106265

承玲 , 许正文 , 韩青 , 史静 , 陆建刚 , 赵云龙 , 李凤英 , 陈敏东 . 超高交联树脂的功能基化及应用研究进展 . 高分子通报 , 2014 , ( 3 ), 23 - 29 .

张全兴 , 陈金龙 , 李爱民 , 杨维本 . 树脂对化工废水中有毒有机化合物的吸附作用机理与技术研究 . 高分子学报 , 2008 , 39 ( 7 ), 651 - 655 .

Peng X. Q. ; Yang P. P. ; Dai K. ; Chen Y. ; Chen X. C. ; Zhuang W. ; Ying H. J. ; Wu J. L. Synthesis, adsorption and molecular simulation study of methylamine-modified hyper-cross-linked resins for efficient removal of citric acid from aqueous solution . Sci. Rep. , 2020 , 10 ( 1 ), 9623 . doi: 10.1038/s41598-020-66592-8 http://dx.doi.org/10.1038/s41598-020-66592-8

Moradi M. R. ; Torkashvand A. ; Ramezanipour Penchah H. ; Ghaemi A. Amine functionalized benzene based hypercrosslinked polymer as an adsorbent for CO 2 /N 2 adsorption . Sci. Rep. , 2023 , 13 ( 1 ), 9214 . doi: 10.1038/s41598-023-36434-4 http://dx.doi.org/10.1038/s41598-023-36434-4

Gan Y. Q. ; Chen G. ; Sang Y. F. ; Zhou F. ; Man R. L. ; Huang J. H. Oxygen-rich hyper-cross-linked polymers with hierarchical porosity for aniline adsorption . Chem. Eng. J. , 2019 , 368 , 29 - 36 . doi: 10.1016/j.cej.2019.02.164 http://dx.doi.org/10.1016/j.cej.2019.02.164

刘云鸿 , 彭新艳 . 新型蛋白结合类毒素血液灌流吸附剂的制备及吸附性能 . 高等学校化学学报 , 2021 , 42 ( 6 ), 1952 - 1964 .

Jung J. ; Choi H. ; Hong S. ; Yoon S. J. ; Kim T. H. ; Lee J. Y. ; Hong Y. T. ; So S. Surface-initiated ATRP of glycidyl methacrylate in the presence of divinylbenzene on porous polystyrene-based resins for boron adsorption . Desalination , 2020 , 473 , 114166 . doi: 10.1016/j.desal.2019.114166 http://dx.doi.org/10.1016/j.desal.2019.114166

袁暾 , 单晶 , 郑利萍 , 朱蔚精 , 梁洁 . 部分凝血激活酶时间试验及全血凝固时间试验方法用于评价心血管系统外部接入器械凝血性能的研究 . 生物医学工程学杂志 , 2009 , 26 ( 4 ), 811 - 814 .

Law R. V. ; Sherrington D. C. ; Snape C. E. ; Ando I. ; Kurosu H. Solid-state 13 C MAS NMR studies of hyper-cross-linked polystyrene resins . Macromolecules , 1996 , 29 ( 19 ), 6284 - 6293 . doi: 10.1021/ma951606o http://dx.doi.org/10.1021/ma951606o

Law R. V. ; Sherrington D. C. ; Snape C. E. Quantitative solid state 13 C NMR studies of highly cross-linked poly(divinylbenzene) resins . Macromolecules , 1997 , 30 ( 10 ), 2868 - 2875 . doi: 10.1021/ma9616470 http://dx.doi.org/10.1021/ma9616470

Views

583

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

Xin-yan Peng

Yun-hong Liu

Related Institution

No data

AI问答

Postal code：100190
Tel：010-62588927 Email：gfzxb@iccas.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05002797号-8 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰