Efficient Self-Assembly and Monodispersity Control of 24-Helix DNA Origami Structures

Xiao-fang Guo; Ya Wang; Tong-kai Zhan; Wei-na Fang

doi:10.11777/j.issn1000-3304.2025.25092

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Efficient Self-Assembly and Monodispersity Control of 24-Helix DNA Origami Structures

更新时间：2025-06-27

- Efficient Self-Assembly and Monodispersity Control of 24-Helix DNA Origami Structures
- Acta Polymerica Sinica Pages: 1-9(2025)
- 作者机构：
  
  华东师范大学化学与分子工程学院上海 200241
- 作者简介：
  
  Wei-na Fang, E-mail: wnfang@chem.ecnu.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2025.25092
  CLC：
- CSTR：32057.14.GFZXB.2025.7424
- Received：07 April 2025，
  
  Accepted：23 May 2025，
  
  Published Online：27 June 2025，
- 稿件说明：
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郭小芳, 王雅, 詹童凯, 方维娜. 24螺旋DNA折纸结构的高效自组装与单分散性调控研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.25092

Guo, X. F.; Wang, Y.; Zhan, T. K.; Fang, W. N. Efficient self-assembly and monodispersity control of 24-helix DNA origami structures. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2025.25092
郭小芳, 王雅, 詹童凯, 方维娜. 24螺旋DNA折纸结构的高效自组装与单分散性调控研究. 高分子学报, doi: 10.11777/j.issn1000-3304.2025.25092 DOI： CSTR： 32057.14.GFZXB.2025.7424.

Guo, X. F.; Wang, Y.; Zhan, T. K.; Fang, W. N. Efficient self-assembly and monodispersity control of 24-helix DNA origami structures. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2025.25092 DOI： CSTR： 32057.14.GFZXB.2025.7424.

摘要

DNA折纸术利用单链DNA骨架与订书钉链的精确退火，可高效构建具有原子级精度的二维/三维纳米结构，在生物传感、纳米光子学和靶向

药物递送等领域展现出重要应用前景. 然而，折纸结构间的非特异性聚集问题严重制约了其功能化应用. 本研究以24螺旋(24-helix bundles，24HB) DNA折纸为模型体系，通过优化Mg

浓度和退火梯度等合成条件，成功制备出结构完整性达96%的目标产物，进一步探究了末端单链刷修饰和骨架环设计两种策略对24 HB DNA折纸单分散性的影响. 结果表明，在24HB DNA折纸单末端修饰聚T (4 nt或7 nt)单链刷可显著提升体系单分散性，其中7 nt单链刷的分散效果优于4 nt. 双端骨架环设计(单分散性72%)优于单端骨架环设计(42%)，其效果与7 nt单末端聚T单链刷(78%)相当. 最后，在双端骨架环的基础上，构建骨架环-末端单链刷复合策略，使单分散性达到87%. 该研究不仅建立了DNA折纸高纯度制备的标准化方案，更为功能性DNA纳米器件的精准组装提供了普适性设计准则.

Abstract

DNA origami technology enables the precise assembly of two- and three-dimensional nanostructures with atomic-level accuracy through the programmed annealing of a single-stranded DNA scaffold and staple strands

demonstrating significant potential in biosensing

nanophotonics

and targeted drug delivery. However

nonspecific aggregation among origami structures severely limits their functionality. In this study

we employed the 24-helix bundle (24HB) DNA origami as a model system and successfully achieved a structurally intact product yield of 96% by optimizing the synthesis conditions

including Mg

concentration and annealing gradient. Furthermore

we investigated the effects of two strategies—terminal single-strand brush modification and scaffold

loop design—on the monodispersity of the 24HB DNA origami. The results demonstrated that modifying the 24HB DNA origami with PolyT (4 nt or 7 nt) single-strand brushes at one terminus significantly improved monodispersity

with the 7 nt brush exhibiting superior dispersion efficiency compared with the 4 nt variant. Additionally

a double-end scaffold loop design (72% monodispersity) outperformed the single-end design (42%) and was comparable to the 7 nt single-end PolyT brush modification (78%). Finally

by integrating a double-end scaffold loop with terminal single-strand brushes

we developed a composite strategy that achieved 87% monodispersity. This study establishes a standardized protocol for high-purity DNA origami fabrication and provides universal design principles for the precise assembly of functional DNA nanodevices.

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Related Author

Wei-na Fang

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Related Institution

Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences

Univerity of Chinese Academy of Sciences

School of Emergent Soft Matter, Guangdong Provincial Key Laboratory of Functional and Intelligent Hybrid Materials and Devices, South China University of Technology

Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology

School of Materials Science and Engineering, Tianjin University

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