Self-assembly Behavior of Polypeptides under Microfluidic Shear Condition

Qian-qing Li; Liang Gao; Jia-ping Lin; Chun-hua Cai

doi:10.11777/j.issn1000-3304.2025.25108

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Self-assembly Behavior of Polypeptides under Microfluidic Shear Condition

Research Article | 更新时间：2025-10-29

- Self-assembly Behavior of Polypeptides under Microfluidic Shear Condition
- Acta Polymerica Sinica Vol. 56, Issue 11, Pages: 2033-2041(2025)
- 作者机构：
  
  华东理工大学材料科学与工程学院上海 200237
- 作者简介：
  
  Liang Gao, E-mail: lianggao@ecust.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2025.25108
  CLC：
- CSTR：32057.14.GFZXB.2025.7437
- Received：23 April 2025，
  
  Accepted：06 June 2025，
  
  Published Online：14 August 2025，
  
  Published：20 November 2025
- 稿件说明：
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李芊晴, 高梁, 林嘉平, 蔡春华. 微流控剪切条件下的聚肽自组装行为. 高分子学报, 2025, 56(11), 2033-2041

Li, Q. Q.; Gao, L.; Lin, J. P.; Cai, C. H. Self-assembly behavior of polypeptides under microfluidic shear condition. Acta Polymerica Sinica, 2025, 56(11), 2033-2041
李芊晴, 高梁, 林嘉平, 蔡春华. 微流控剪切条件下的聚肽自组装行为. 高分子学报, 2025, 56(11), 2033-2041 DOI： 10.11777/j.issn1000-3304.2025.25108. CSTR： 32057.14.GFZXB.2025.7437.

Li, Q. Q.; Gao, L.; Lin, J. P.; Cai, C. H. Self-assembly behavior of polypeptides under microfluidic shear condition. Acta Polymerica Sinica, 2025, 56(11), 2033-2041 DOI： 10.11777/j.issn1000-3304.2025.25108. CSTR： 32057.14.GFZXB.2025.7437.

摘要

微流控通道中独特的剪切环境为构筑复杂纳米结构带来了新的机遇. 本研究设计了一种环型微流控通道，利用剪切作用使刚性聚肽形成与溶液自组装不同的扭曲状聚集体. 通过调控剪切速率和含水量，结合圆二色谱(CD)与同步辐射广角X射线衍射测试(WAXS)，探究了聚(

-苄基-

L-

谷氨酸酯) (PBLG)形成扭曲状聚集体的机理. 在选择性溶剂(水)的剪切作用下，聚肽链间距减小、链有序排列更紧密，同时其

-螺旋构象向PPⅡ-螺旋构象转变，这使聚集体产生内扭力，因而在剪切条件下发生形貌转变，形成新颖的扭曲状形貌结构. 研究结果为定向设计多级结构提供了新策略.

Abstract

The unique shear environment in microfluidic channels offers new opportunities for constructing complex nanostructures. In this study

a ring-shaped microfluidic channel was designed to utilize shear forces to induce rigid polypeptides for forming twisted aggregates that are different from those formed by solut

ion self-assembly. By examining the effects of shear rate and water content

and combining circular dichroism (CD) and synchrotron wide-angle X-ray scattering (WAXS) tests

the formation mechanism of twisted aggregate of poly(

-benzyl-

-glutamate) (PBLG) was revealed. Under the shear of a selective solvent (water)

the distance between polypeptide chains decreases

and the chain arrangement becomes more ordered and compact. Meanwhile

the α-helix conformation of the polypeptide transforms into PPⅡ-helix conformation

which generates an internal twisted force in the aggregates

forming a novel twisted aggregate morphology. The research results can provide a new strategy for the directed design of hierarchical structures.

关键词

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references

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