The poria cocos mycelia samples were cultured in media at 25℃ for one week.Four polysaccharides coded as PCM1

PCM2

PCM3 and PCM4 were isolated from the poria cocos mycelia by using 0.9% NaCl aqueous solution

hot water

0.5mol/L NaOH aqueous solution and 88% formic acid

respectively.The components and structure of the polysaccharides were analyzed by infrared

gas chromatography

high performance liquid chromatography and 13C nuclear magnetic resonance.The results show that PCM1 and PCM2 consist of D-rhamnose

D-fucose

D-xylose

D-mannose

D-galactose

D-glucose and D-glucuronic acid

and the D-glucose content increases with the progress of isolation.PCM3 is mainly linear β-(1→3)-D-glucan and the major fraction of polysaccharides fron the poria cocos mycelia (yield 55.8%).PCM4 is composed of D-glucose with a few glucuronic acids.It is summarized that the coomponent and structure of the polysaccharides from poria cocos mycelia are similar to those from poria cocos sclerotium.