脂质体的特异性识别与单颗粒电化学检测

王克青; 李自若; 费进波; 王晨蕾; 崔巍; 赵洁; 李峻柏

doi:10.11777/j.issn1000-3304.2021.21083

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脂质体的特异性识别与单颗粒电化学检测

研究论文 | 更新时间：2022-09-30

- 脂质体的特异性识别与单颗粒电化学检测
- Specific Molecular Recognition of Liposome Nanoparticles and Single-particle Electrochemical Detection
- 高分子学报 2021年52卷第8期页码：1024-1031
- 作者机构：
  
  1.中国科学院化学研究所胶体、界面与化学热力学重点实验室北京 100190
  2.中国科学院大学北京 100049
- 作者简介：
  
  E-mail: jbli@iccas.ac.cn
- 基金信息：
  
  国家自然科学基金(基金号 ┣21961142022;21872150);22072160┫
- DOI：10.11777/j.issn1000-3304.2021.21083
  中图分类号：
- 纸质出版日期：2021-08-20，
  
  网络出版日期：2021-06-17，
  
  收稿日期：2021-03-14，
  
  修回日期：2021-04-07，
扫描看全文
王克青,李自若,费进波等.脂质体的特异性识别与单颗粒电化学检测[J].高分子学报,2021,52(08):1024-1031.

Wang Ke-qing,Li Zi-ruo,Fei Jin-bo,et al.Specific Molecular Recognition of Liposome Nanoparticles and Single-particle Electrochemical Detection[J].ACTA POLYMERICA SINICA,2021,52(08):1024-1031.
王克青,李自若,费进波等.脂质体的特异性识别与单颗粒电化学检测[J].高分子学报,2021,52(08):1024-1031. DOI： 10.11777/j.issn1000-3304.2021.21083.

Wang Ke-qing,Li Zi-ruo,Fei Jin-bo,et al.Specific Molecular Recognition of Liposome Nanoparticles and Single-particle Electrochemical Detection[J].ACTA POLYMERICA SINICA,2021,52(08):1024-1031. DOI： 10.11777/j.issn1000-3304.2021.21083.

摘要

脂质体载药系统已在许多疾病的治疗中发挥重要作用. 体外构建并模拟脂质体与生物膜特异性识别相互作用，对于探索脂质体在生物体内的稳定性，开发新型高效和智能化药物递送系统具有重要的现实意义. 本文通过分子组装技术构筑了稳定的磷脂囊泡-脂质体，利用微加工技术制备金微电极，然后在其表面进行亲合素修饰，借助电化学工作站检测脂质体单颗粒的碰撞行为，以此建立一种检测脂质体在体外和生物体内存在的技术平台. 实验证明，在脂质体中引入生物素分子，通过与微电极表面的亲合素分子产生特异性识别，能显著增加单颗粒碰撞的频率和强度. 这些定量的数据为研究脂质体与生物膜动态互作提供了十分有效和准确的检测手段，为验证脂质体载药体系在生物内的稳定性和释放速率建立了新方法.

Abstract

Liposomes delivery drug systems show obvious advantages in the treatment of many diseases. Constructing new types of liposome based drugs is of great importance to develop more efficient novel drug delivery systems. Herein

the present work demonstrated that Au microelectrode was chemically modified with avidin through Au-S bond (Avidin-S-Au microelectrode) and liposomes modified with the biotin (Biotin-liposome) were constructed through molecular assembly. Furthermore

an electrochemical detection device was constructed to recognize liposomes through single nanoparticle collision. Compared with pure liposomes

biotin modified liposome displayed a higher collision frequency and intensity with Avidin-S-Au microelectrode. Importantly

with the increasing concentration of biotin-liposome

the collision frequency increases as well. Taking the single liposome collision to the gold microelectrode into account

one can obtain quantitative information of the liposome size and concentration through the frequency and intensity of single nanoparticle collision. These findings represent an unusual case to achieve high sensitivity detection of individual liposomes with poor electrochemical activity and at very low concentration. The present work creates a simple and effective way to detect the stability of liposomes and their interaction with biomembranes.

关键词

脂质体超分子组装单颗粒碰撞电化学检测分子识别

Keywords

LiposomeMolecular assemblySingle particle collisionElectrochemical detectionMolecular recognition

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