光交联交替层状自组装(LBL)抗菌膜的制备及其性能研究

王学斌; 薛源; 周耀武; 陈声毅; 包春燕; 朱麟勇

doi:10.11777/j.issn1000-3304.2021.21182

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光交联交替层状自组装(LBL)抗菌膜的制备及其性能研究

研究论文 | 更新时间：2024-10-08

- 光交联交替层状自组装(LBL)抗菌膜的制备及其性能研究
- Preparation and Characterization of Photo-crosslinked Layer-by-Layer (LBL) Self-assembled Antibacterial Films
- 高分子学报 2022年53卷第2期页码：133-144
- 作者机构：
  
  上海市功能性材料化学重点实验室华东理工大学化学与分子工程学院上海 200237
- 作者简介：
  
  E-mail: baochunyan@ecust.edu.cn
- 基金信息：
  
  上海市自然科学基金项目(基金号 21ZR1415500)
- DOI：10.11777/j.issn1000-3304.2021.21182
  中图分类号：
- 纸质出版日期：2022-02-20，
  
  网络出版日期：2021-11-15，
  
  收稿日期：2021-07-05，
  
  录用日期：2021-09-13
- 稿件说明：
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王学斌,薛源,周耀武等.光交联交替层状自组装(LBL)抗菌膜的制备及其性能研究[J].高分子学报,2022,53(02):133-144.

Wang Xue-bin,Xue Yuan,Zhou Yao-wu,et al.Preparation and Characterization of Photo-crosslinked Layer-by-Layer (LBL) Self-assembled Antibacterial Films[J].ACTA POLYMERICA SINICA,2022,53(02):133-144.
王学斌,薛源,周耀武等.光交联交替层状自组装(LBL)抗菌膜的制备及其性能研究[J].高分子学报,2022,53(02):133-144. DOI： 10.11777/j.issn1000-3304.2021.21182.

Wang Xue-bin,Xue Yuan,Zhou Yao-wu,et al.Preparation and Characterization of Photo-crosslinked Layer-by-Layer (LBL) Self-assembled Antibacterial Films[J].ACTA POLYMERICA SINICA,2022,53(02):133-144. DOI： 10.11777/j.issn1000-3304.2021.21182.

摘要

针对静电超分子作用的交替层状自组装(LBL)膜稳定性差、易分解和难以实现应用的问题，设计合成了一种光交联型抗菌LBL多层膜. 该LBL多层膜由修饰有光敏邻硝基苄醇分子的透明质酸(HANB)与季铵化的壳聚糖(ACS)通过静电作用在基片上交替沉积而成. 光照后，HANB上的邻硝基苄醇分子产生活性醛基，与邻近壳聚糖上的氨基发生亚胺偶联反应，实现了LBL多层膜的光交联. 实验证明交联后的LBL膜具有很好的稳定性，能在不同pH值盐溶液中长期保持膜的完整性. 壳聚糖的抗菌特性赋予LBL膜良好的抗菌性能，亲水性生物大分子骨架的选取赋予LBL膜良好的细胞相容性. 与其他化学交联方法相比，邻硝基苄醇光交联策略无需任何引发剂或添加剂的引入，具有更好的生物相容性，为医疗植入物表面抗菌涂层的构建提供新方法.

Abstract

Bacteria are one

of the oldest life forms on the earth. They have strong adaptability to various environments and can be seen almost everywhere on the earth. Bacteria will adhere to different surfaces and then reproduce to form dense aggregates or biofilms with a thickness of several microns to tens of centimeters

which will seriously affect the properties of materials

especially for medical devices. Bacterial adhesion and biofilm formation on these devices are an important reason for the failure of medical implantation and the formation of large-area medical infection. Therefore

researchers are focusing on developing antibacterial surface coatings to eliminate or prevent the initial adhesion of bacteria and biofilm formation on the surface of materials. Alternating layered self-assembly (LBL) film

as the most popular coating technology

is applicable to the surface of various materials. Due to its advantages of simple operation

high controllability and easy functionalization

LBL film is widely used in separation technology

sensor

controlled drug release

surface modification of biomaterials and so on. However

its inherent shortcomings

such as poor stability

easy decomposition

etc.

greatly limit their applications. To solve the above problems

a kind of photo crosslinking antibacterial LBL membrane was designed and prepared by alternating deposition of photosensitive

-nitrobenzyl alcohol modified hyaluronic acid (HANB) and quaternized chitosan (ACS) on the glass substrate through electrostatic interaction. The photoligation reaction of

-nitrobenzyl alcohol to amine of chitosan enables the crosslinking of the assembled polyelectrolyte membrane

which stabilizes and maintains the integrity of the membrane for a long time in salt solutions with different pH values. The antibacterial property of chitosan endows LBL membrane with good antibacterial property and strong electrostatic supramolecular interactions with HANB. Cytotoxicity analysis confirms the cytocompatibil

ity of the LBL membranes. Antibacterial experiments show that the non-photocrosslinked LBL membrane shows higher release-sterilization due to the release of quaternized chitosan to the solution. However

the photocrosslinked LBL membrane shows better contact-sterilization due to the membrane stability

which is more friendly to the environment. Compared with other chemical crosslinking methods

-nitrobenzyl alcohol photocrosslinking strategy has better biocompatibility without the introduction of any initiator or additive

which provides a new idea for the construction of antibacterial coating on the surface of medical implants.

关键词

交替层状自组装膜抗菌涂层光交联细胞相容性

Keywords

Alternating layered self-assembly filmAntibacterial coatingPhotocrosslinkCytocompatibility

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