益生菌/黑磷纳米片复合材料在肠炎治疗中的应用

姚炜钦; 孙云霞; 陈巍海; 张先正

doi:10.11777/j.issn1000-3304.2024.0207;

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益生菌/黑磷纳米片复合材料在肠炎治疗中的应用

研究论文 | 更新时间：2024-11-07

- 益生菌/黑磷纳米片复合材料在肠炎治疗中的应用
- Application of Engineered Probiotic-nanosystem for the Treatment of Enteritis
- 高分子学报 2024年页码：1-11
- 作者机构：
  
  武汉大学生物医用高分子材料教育部重点实验室/化学与分子科学学院武汉430072
- 作者简介：
  
  E-mail: chenweihai@whu.edu.cn
  xz-zhang@whu.edu.cn
- 基金信息：
  
  国家重点基础研究发展计划(2022YFB3804600);国家自然科学基金(基金号 ┣52273301;52073218);22135005┫
- DOI：10.11777/j.issn1000-3304.2024.0207;
  中图分类号：
- CSTR：32057.14.GFZXB.2024.7295
- 网络出版日期：2024-10-07，
  
  收稿日期：2024-07-29，
  
  录用日期：2024-09-03
- 稿件说明：
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姚炜钦, 孙云霞, 陈巍海, 张先正. 益生菌/黑磷纳米片复合材料在肠炎治疗中的应用. 高分子学报, doi: 10.11777/j.issn1000-3304.2024.0207

Yao, W. Q.; Sun, Y. X.; Chen, W. H.; Zhang, X. Z. Application of engineered probiotic-nanosystem for the treatment of enteritis. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2024.0207
姚炜钦, 孙云霞, 陈巍海, 张先正. 益生菌/黑磷纳米片复合材料在肠炎治疗中的应用. 高分子学报, doi: 10.11777/j.issn1000-3304.2024.0207 DOI： 10.11777/j.issn1000-3304.2024.0207;. CSTR： 32057.14.GFZXB.2024.7295.

Yao, W. Q.; Sun, Y. X.; Chen, W. H.; Zhang, X. Z. Application of engineered probiotic-nanosystem for the treatment of enteritis. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2024.0207 DOI： 10.11777/j.issn1000-3304.2024.0207;. CSTR： 32057.14.GFZXB.2024.7295.

摘要

长期暴露在重金属离子环境中或误食过量重金属离子都会导致重金属离子在肠道富集，进而引发肠道部位发生持续性的炎症，严重的甚至会导致其他器官发生病变. 因此，在肠道受损部位高效且持续性的清除重金属离子是治疗这一类疾病的关键. 为此，本研究设计、制备了一种益生菌/黑磷纳米片复合材料

实现了肠道部位重金属离子的高效且持续性的清除，并且能够有效修复受损肠道. 通过静电相互作用将氨基封端的聚乙二醇(NH

-PEG

2000

-NH

)修饰在黑磷纳米片(black phosphorus nanosheets，BPs)表面，随后聚乙二醇化的黑磷纳米片通过酰胺缩合反应与鼠李糖乳杆菌(

Lactobacillus rhamnosus

GG，LGG)结合，构建BPs-LGG益生菌/黑磷纳米片复合材料. 在重金属离子诱导的肠炎小鼠模型中，口服递送BPs-LGG后，鼠李糖乳杆菌能够将黑磷纳米片靶向递送至肠道受损部位并长期定植，从而在肠道受损部位持续、高效地清除重金属离子并缓解炎症. 该治疗策略具有良好的生物安全性，为治疗重金属离子引起的肠道疾病提供了一种安全、有效的思路.

Abstract

Long-term exposure to heavy metal ions or accidentally consuming excessive amounts of heavy metal ions can induce the accumulation of heavy metal ions in the intestines

leading to chronic inflammation in the intestinal area and even causing

dysfunction of other organs. Therefore

efficient and sustained removal of heavy metal ions from damaged intestinal tissue is crucial for treating such diseases. Here

an engineered probiotic-nanosystem was designed for efficiently and sustainably removing heavy metal ions from the intestinal tract and effectively repairing damaged intestinal tissues. Firstly

-poly(ethylene glycol)

2000

-NH

(NH

-(PEG

2000

)-NH

) was modified on the surface of black phosphorus nanosheets (BPs) through electrostatic interactions. Then

the NH

-PEG

2000

-NH

functionalized BPs were further coupled with

Lactobacillus rhamnosus

GG (LGG) through the amide condensation reaction to form the BPs-LGG probiotic-nanosystem. After oral delivery of the BPs-LGG into the enteritis mouse model induced by heavy metal ion

LGG as the carrier of BPs

could effectively deliver BPs into intestinal area and colonize in the impaired parts of intestines

thus establishing a long-term colonization of BPs. These probiotic-nanosystem achieved a sustained and efficient removal of heavy metal ions and alleviated inflammatory responses in the impaired area of intestines. This therapeutic strategy has an extremely outstanding biocompatibility and provides a promising approach for treating intestinal diseases caused by heavy metal ions

showing the promising potential for potentiating therapeutic efficacy of enteritis.

关键词

重金属中毒益生菌黑磷纳米片复合材料肠炎

Keywords

Heavy metal poisoningProbioticBlack phosphorus nanosheetComposite materialEnteritis

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