PCL-b-PEG with different composition and functional groups were used to prepare microspheres with different surface properties by means of the double emulsion solvent evaporation method. Then

the biomineralization of microspheres was performed in the 1.5 simulated body fluid (1.5SBF). The effects of the hydrophilicity

roughness and functional groups of microsphere surface on the formation of apatite were studied. It was found that the higher molar ratio of PEG to PCL leads to rougher and more hydrophilic microsphere surface

which results in the formation of more apatite. The functional groups were found to have great influence on the formation and distribution of apatite on the surface of microspheres. Apatite was distributed homogeneously on the surface of PCL20K-b-PEG1K-COOH microspheres. Moreover

the amount of apatite on microsphere surface increased with the mineralization time. The biomineralization had an initial period of ca. 4 days for apatite nucleation

followed by normal gradual growth. The results of this work indicate that the surface properties of microspheres such as hydrophilicity

roughness and functional groups as well as the biomineralizaiton time could be used to control the apatite formation on microsphere surface. These results are expected to be used in the construction of tissue engineering scaffolds.