Protein Refolding Assisted by Thermosensitive Complex Micelles

Hai-xia Zhang; Yi-qing Song; Ying-li An; Ying Wang; Lin-qi Shi

doi:10.11777/j.issn1000-3304.2014.14096

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Protein Refolding Assisted by Thermosensitive Complex Micelles

Research Article | 更新时间：2021-03-19

- Protein Refolding Assisted by Thermosensitive Complex Micelles
- Acta Polymerica Sinica Issue 12, Pages: 1561-1567(2014)
- 作者机构：
  
  功能高分子材料教育部重点实验室南开大学高分子化学研究所天津化学化工协同创新中心,天津,300071
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2014.14096
  CLC：
- Published：20 December 2014，
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Hai-xia Zhang, Yi-qing Song, Ying-li An, Ying Wang, Lin-qi Shi. Protein Refolding Assisted by Thermosensitive Complex Micelles. [J]. Acta Polymerica Sinica (12):1561-1567(2014)
DOI：

Hai-xia Zhang, Yi-qing Song, Ying-li An, Ying Wang, Lin-qi Shi. Protein Refolding Assisted by Thermosensitive Complex Micelles. [J]. Acta Polymerica Sinica (12):1561-1567(2014) DOI： 10.11777/j.issn1000-3304.2014.14096.

摘要

提出了一种使用复合胶束模拟分子伴侣来辅助蛋白质折叠的方法.制备了3种由不同比例的嵌段共聚物聚乳酸-b-聚(N-异丙基丙烯酰胺)(PLA-b-PNIPAM)与聚乳酸-b-聚乙二醇(PLA-b-PEG)组成的温敏性的复合胶束(mixed-shell polymeric micelles

MSPMs)

通过动态光散射考察了复合胶束在37 ℃以及在变性剂存在下的稳定性.以碳酸酐酶(CAB)和脂肪酶(Lipase)作为模型蛋白

研究了MSPM辅助蛋白质折叠的能力.结果表明

在高浓度变性剂存在下

PNIPAM仍具有温敏性

并且摩尔比为1:1的PLA-b-PNIPAM和PLA-b-PEG制备的MSPM具有非常好的稳定性.不同比例的MSPM对于CAB和Lipase都具有辅助复性效果

远远高于没有MSPM时的自发复性.其中

摩尔比为1:1的MSPM使CAB的复性率高达92.1%

Lipase的复性率也达到50%.

Abstract

A new method for assisting protein folding by using thermosensitive mixed-shell polymeric micelles (MSPMs) as artificial chaperone was introduced.Three kinds of novel thermosensitive MSPMs with different ratios of PLA-b-PEG and PLA-b-PNIPAM were prepared.The stability of MSPMs at different concentrations of guanidine hydrochloride (GuHCl) and urea or at 37 ℃ was characterizaed by dynamic light scattering (DLS).Carbonic anhydrase B (CAB) and lipase were chosen as model proteins to illustrate the effect of protein refolding assisted by thermosensitive MSPMs.The results showed that the thermosensitive property of PNIPAM was maintained and the stability of MSPM with PLA-b-PNIPAM/PLA-b-PEG ratio of 1:1 was very good even under conditions of high concentration of denaturant or 37 ℃.Furthermore

all the three kinds of MSPMs with ratio of 2:1

1:2 and 1:1 can promote the refolding of denatured CAB and lipase

which are much higher than the yields of spontaneous refolding.The MSPM with molar ratio of 1:1 can enhance the refolding yield of CAB to reach as high as 92.1% and that of lipase to reach 50%.

关键词

温敏性聚合物复合胶束蛋白质折叠人工伴侣疏水作用

Keywords

Thermosensitive polymerMixed-shell polymeric micellesProtein foldingArtificial chaperoneHydrophobic interaction

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Department of Macromolecular Science, State Key Laboratory of Macromolecular Engineering of Polymers, Fudan University

The Key Laboratory of Space Applied Physics and Chemistry, Ministry of Education and Shaanxi Key Laboratory of Macromolecular Science and Technology, School of Science, Northwestern Polytechnical University, Xi&rsquo

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