Dynamic Spongy Microporous Films to Load Lysozyme for Antibacterial Coating

Wen-xi Lei; Ke-feng Ren; Xia-chao Chen; Mi Hu; Jian Ji

doi:10.11777/j.issn1000-3304.2017.16260

您当前的位置：

首页 >

文章列表页 >

Dynamic Spongy Microporous Films to Load Lysozyme for Antibacterial Coating

Research Article | 更新时间：2021-03-19

- Dynamic Spongy Microporous Films to Load Lysozyme for Antibacterial Coating
- Acta Polymerica Sinica Issue 5, Pages: 744-751(2017)
- 作者机构：
  
  浙江大学高分子科学与工程学系教育部高分子合成与功能构造教育部重点实验室杭州 310027
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2017.16260
  CLC：
- Published：2017-5，
  
  Received：22 August 2016，
  
  Revised：24 September 2016，
扫描看全文
Wen-xi Lei, Ke-feng Ren, Xia-chao Chen, Mi Hu, Jian Ji. Dynamic Spongy Microporous Films to Load Lysozyme for Antibacterial Coating. [J]. Acta Polymerica Sinica (5):744-751(2017)
DOI：

Wen-xi Lei, Ke-feng Ren, Xia-chao Chen, Mi Hu, Jian Ji. Dynamic Spongy Microporous Films to Load Lysozyme for Antibacterial Coating. [J]. Acta Polymerica Sinica (5):744-751(2017) DOI： 10.11777/j.issn1000-3304.2017.16260.

摘要

以聚丙烯酸（PAA）和聚乙烯亚胺（PEI）为构筑单元，运用层层自组装技术制备了聚电解质多层膜.该多层膜具有独特的动态特点--经酸处理后膜内部形成海绵状通孔结构，该海绵结构在饱和水蒸气的处理下，多孔结构能够闭合，重新回到致密的膜结构.借助该种动态多层膜平台，能够简单有效地通过毛细作用力将溶菌酶负载并固定于多层膜中，为制备基于抗菌蛋白的抗菌涂层提供了新的方法.扫描电镜表征了多层膜动态变化过程，激光共聚焦显微镜表征了溶菌酶在膜内的分布情况，并测定了溶菌酶载入量及其释放动力学.进一步的抗菌测试表明该种抗菌涂层在溶菌酶和PEI的共同作用下可以有效地抑制金黄色葡萄球菌.将多层膜同时负载溶菌酶和乳铁蛋白，提升了涂层对大肠杆菌的杀菌效果.

Abstract

Polyelectrolyte multilayer films were fabricated by layer-by-layer deposition of polyacrylic acid (PAA) and polyethyleneimine (PEI). The (PAA/PEI) films showed unique dynamic properties owning to the high mobility of the polymer molecules:the spongy microporous structure was formed through an acid treatment for 60 min; and the porous structure disappeared under a 100% relative humidity treatment

back to a solid film. Based on this (PAA/PEI) platform

lysozyme was easily loaded into the film via a wicking action in 5 s

and then easily immobilized into the film after 8 h conservation in humid atmosphere

providing therefore a novel approach to prepare antibacterial coating without any specific requirement for the characteristic of the agents. Scanning electron microscope was employed to show the dynamic transformation of film structures

from the original film with 9.5 nm of thickness to spongy sporous film with 68 nm of thickness and back to the solid film of 12 nm after the conservation process. Confocal scanning laser microscope image showed an even distribution of lysozyme in the film with lysozyme labelled by FITC. Loading quantity and the release dynamics of lysozyme were tested utilizing ultraviolet-visible spectrophotometer. Further

gram positive bacteria

S. aureus

and gram negative bacteria

E. coli

were tested in the antibacterial assay. The result illustrated that the coating was able to kill

S. aureus

efficiently

and the antibacterial activity of lysozyme was not affected by such loading and releasing process. To further enhance the bactericidal effect of the coating against gram negative bacteria

lysozyme and lactoferrin

which have synergistic killing effect on gram negative bacteria

were immobilized into the film at the same time and performed a distinct higher antibacterial efficiency against

E. coli

in comparison with the (PEI/PAA)

-lysozyme coating

revealing the attractive potential of this film as antibacterial coatings for medical devices to prevent the hospital-associated infections.

关键词

动态转变多孔海绵结构溶菌酶层层自组装抗菌涂层

Keywords

Dynamic transformationSpongy microporous structureLysozymeLayer-by-layer assemblyAntibacterial coating

references

M Klevens , R Edwards , L Richards , C Horan , P Gaynes , A Pollock , M Cardo . Public Health Rep , 2007 . 122 ( 2 ): 160 - 166 . DOI:10.1177/003335490712200205http://doi.org/10.1177/003335490712200205.

M Cloutier , D Mantovani , F Rosei . Trends Biotechnol , 2015 . 33 ( 11 ): 637 - 652 . DOI:10.1016/j.tibtech.2015.09.002http://doi.org/10.1016/j.tibtech.2015.09.002.

D G Gao , X Y Duan , C Chen , B Lyu , J Z Ma . Ind Eng Chem Res , 2015 . 54 ( 43 ): 10560 - 10567 . DOI:10.1021/acs.iecr.5b02509http://doi.org/10.1021/acs.iecr.5b02509.

I Matai , A Sachdev , P Dubey , U Kumar , B Bhushan , P Gopinath . Colloid Surface B , 2014 . 115 359 - 367 . DOI:10.1016/j.colsurfb.2013.12.005http://doi.org/10.1016/j.colsurfb.2013.12.005.

J Min , D Braatz , T Hammond . Biomaterials , 2014 . 35 ( 8 ): 2507 - 2517 . DOI:10.1016/j.biomaterials.2013.12.009http://doi.org/10.1016/j.biomaterials.2013.12.009.

K Allen , J Donato , H Wang , A Cloud-Hansen , J Davies , J Handelsman . Nat Rev Microbiol , 2010 . 8 ( 4 ): 251 - 259 . DOI:10.1038/nrmicro2312http://doi.org/10.1038/nrmicro2312.

D Alves , O Pereira . Biofouling , 2014 . 30 ( 4 ): 483 - 499 . DOI:10.1080/08927014.2014.889120http://doi.org/10.1080/08927014.2014.889120.

M Simmaco , G Mignogna , D Barra . Biopolymers , 1998 . 47 ( 6 ): 435 - 450 . DOI:10.1002/(SICI)1097-0282(1998)47:6<>1.0.CO;2-Whttp://doi.org/10.1002/(SICI)1097-0282(1998)47:6<>1.0.CO;2-W.

M Ding , L Shao , J Liu , G Xiao , J Chen . Colloid Interface Sci , 2005 . 290 ( 1 ): 102 - 106 . DOI:10.1016/j.jcis.2005.04.029http://doi.org/10.1016/j.jcis.2005.04.029.

S Li , J Mulloor , L Wang , Y Ji , J Mulloor , M Micic , J Orbulescu , M Leblanc . ACS Appl Mater Interfaces , 2014 . 6 ( 8 ): 5704 - 5712 . DOI:10.1021/am500254ehttp://doi.org/10.1021/am500254e.

K Muszanska , J Busscher , A Herrmann , C van der Mei , W Norde . Biomaterials , 2011 . 32 ( 26 ): 6333 - 6341 . DOI:10.1016/j.biomaterials.2011.05.016http://doi.org/10.1016/j.biomaterials.2011.05.016.

S Yuan , D Wan , B Liang , O Pehkonen , Y P Ting , G Neoh , T Kang . Langmuir , 2011 . 27 ( 6 ): 2761 - 74 . DOI:10.1021/la104442fhttp://doi.org/10.1021/la104442f.

Z Tang , Y Wang , P Podsiadlo , N Kotov . Adv Mater , 2007 . 18 ( 24 ): 3203 - 3224.

X C Chen , K F Ren , J H Zhang , D D Li , E Zhao , Z J Zhao , Z K Xu , J Ji . Adv Funct Mater , 2015 . 25 ( 48 ): 7470 - 7477 . DOI:10.1002/adfm.v25.48http://doi.org/10.1002/adfm.v25.48.

D Mendelsohn , J Barrett , V Chan , J Pal , M Mayes , F Rubner . Langmuir , 2000 . 16 ( 11 ): 5017 - 5023 . DOI:10.1021/la000075ghttp://doi.org/10.1021/la000075g.

Y Li , X Wang , J Q Sun . Chem Soc Rev , 2012 . 41 ( 18 ): 5998 - 6009 . DOI:10.1039/c2cs35107bhttp://doi.org/10.1039/c2cs35107b.

K F Ren , Y X Wang , J Ji , Q K Lin , J C Shen . Colloid Surface B , 2005 . 46 ( 2 ): 63 - 69 . DOI:10.1016/j.colsurfb.2005.09.004http://doi.org/10.1016/j.colsurfb.2005.09.004.

A Lichter , J van Vliet , F Rubner . Macromolecules , 2009 . 42 ( 22 ): 8573 - 8586 . DOI:10.1021/ma901356shttp://doi.org/10.1021/ma901356s.

G Kim , G Park . Biotechnol Progr , 2006 . 22 ( 4 ): 1108 - 1113 . DOI:10.1021/bp060039thttp://doi.org/10.1021/bp060039t.

Y Kacar , Y Arica . Food Chem , 2001 . 75 ( 3 ): 325 - 332 . DOI:10.1016/S0308-8146(01)00208-4http://doi.org/10.1016/S0308-8146(01)00208-4.

A Lichter , F Rubner . Langmuir , 2009 . 25 ( 13 ): 7686 - 7694 . DOI:10.1021/la900349chttp://doi.org/10.1021/la900349c.

W X Lei , X C Chen , M Hu , H Chang , H Xu , K F Ren , J Ji . J Mater Chem B , 2016 . 4 ( 38 ): 6358 - 6365 . DOI:10.1039/C6TB01967Fhttp://doi.org/10.1039/C6TB01967F.

A Zdybicka-Barabas , S Staczek , P Mak , K Skrzypiec , E Mendyk , M Cytrynska . Bba-Biomembranes , 2013 . 1828 ( 6 ): 1449 - 1456 . DOI:10.1016/j.bbamem.2013.02.004http://doi.org/10.1016/j.bbamem.2013.02.004.

T Ellison , J Giehl . J Clin Invest , 1991 . 88 ( 4 ): 1080 - 1091 . DOI:10.1172/JCI115407http://doi.org/10.1172/JCI115407.

Hongfang Wang , Yi Liu , Zhongfeng Yao , Ruitong Dai , Xingmin Li , Wenjie Yan . The Processing of Agricultural Products , 2011 . ( 7 ): 36 - 40 . http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm.

王洪芳 , 刘毅 , 姚中峰 , 戴瑞彤 , 李兴民 , 阎文杰 . 农产品加工学刊 , 2011 . ( 7 ): 36 - 40 . http://www.cnki.com.cn/Article/CJFDTOTAL-SYQY201603027.htm.

更多指标>

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning

Preparation and Characterization of Photo-crosslinked Layer-by-Layer (LBL) Self-assembled Antibacterial Films

Fabrication of (Heparin/Chitosan Oligosaccharides/Pluronic) Multilayer Films via Electrostatic Layer-by-Layer Assembly

Related Author

No data

Related Institution

College of Chemistry and Chemical Engineering, Qingdao University

Materials Science and Engineering, Shandong University of Technology

Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology

School of Materials Science and Engineering, Shanghai University

Key Laboratory of Marine Materials and Related Technologies, Zhejiang Key Laboratory of Marine Materials and Protective Technologies, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences

Postal code：100190
Tel：010-62588927 Email：gfzxb@iccas.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05002797号-8 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰