Preparation of Stimuli Responsive Nanoparticles Using Coaxial Electrospray Template Removal Method and Its Application as Drug Delivery System

Hua-feng Dai; Jia-ming Chen; Li-qun Wang

doi:10.11777/j.issn1000-3304.2017.17036

您当前的位置：

首页 >

文章列表页 >

Preparation of Stimuli Responsive Nanoparticles Using Coaxial Electrospray Template Removal Method and Its Application as Drug Delivery System

Research Article | 更新时间：2021-01-26

- Preparation of Stimuli Responsive Nanoparticles Using Coaxial Electrospray Template Removal Method and Its Application as Drug Delivery System
- Acta Polymerica Sinica Issue 12, Pages: 1947-1954(2017)
- 作者机构：
  
  1.浙江大学高分子科学与工程学系杭州 310027
  2.浙江大学高分子合成与功能构造教育部重点实验室杭州 310027
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2017.17036
  CLC：
- Published：20 December 2017，
  
  Received：3 March 2017，
  
  Revised：9 May 2017，
扫描看全文
Hua-feng Dai, Jia-ming Chen, Li-qun Wang. Preparation of Stimuli Responsive Nanoparticles Using Coaxial Electrospray Template Removal Method and Its Application as Drug Delivery System. [J]. Acta Polymerica Sinica (12):1947-1954(2017)
DOI：

Hua-feng Dai, Jia-ming Chen, Li-qun Wang. Preparation of Stimuli Responsive Nanoparticles Using Coaxial Electrospray Template Removal Method and Its Application as Drug Delivery System. [J]. Acta Polymerica Sinica (12):1947-1954(2017) DOI： 10.11777/j.issn1000-3304.2017.17036.

摘要

利用"同轴电喷-去模板技术"制备了载药效率接近100%，且具有核-壳结构和氧化还原响应性的药物控释微球.首先通过同轴电喷技术，制备了以聚乙二醇（PEG）为壳，主链含多个二硫键的聚醚氨酯（PEU）为核的微球.通过"去模板"方法，脱去PEG层，可以使所制备的微球的尺寸从微米减小到纳米尺度.在含二硫苏糖醇（DTT）的媒介中，PEU可以快速降解.体外释药的结果显示，在含谷胱甘肽（GSH）的媒介中，载药纳米微球可在12 h内将其所包载的药物的80%释放出，具有显著的氧化还原响应性控制释放药物的特征.

Abstract

Redox-responsive microparticles with core-shell structure were prepared by the coaxial electrospray technique. Poly(ether urethane) (PEU) with multiple-disulfide bonds in the main chain was synthesized

via

a facile one-pot method. It was found that the number average molecular weight of the resultant polymer decreased from 3.1×10

to 6.6×10

in 8 h in the presence of DL-dithiothreitol (DTT). The microparticles with PEU as the core and polyethylene glycol (PEG) as the shell were prepared by the coaxial electrospray technique. The results of the microscopies (the scanning electron microscopy and laser scanning confocal microscopy) showed that the constructed microparticles were spherical

uniform and with a distinct core-shell structure. It was also found that the mean diameter of the prepared microparticles decreased to less than 100 nm when the PEG shell was removed. Dynamic light scattering results showed that the hydrodynamic mean diameter of the particles after PEG template removal was about 112 nm. The spherical nanoparticles could be completely decomposed when they were placed in phosphate buffer saline (PBS) solution at pH=7.4 + 10 mmol/L L-glutathione (GSH) for 24 h at 37℃. In contrast

the shape of the spherical nanoparticles remained unchanged in PBS without GSH after 24 h. The doxorubicin (DOX) loaded nanoparticles were also prepared with the coaxial electrospray technique

and the results showed that the drug-loading efficiency was nearly 100%. Meanwhile

under the condition of GSH

the content of the cumulative drug release could be up to 80% within 12 h at 37℃

in contrast to the case that only 50% of the loaded DOX could release within 12 h in the absence of GSH. This study shows that the prepared nano drug delivery system has the characteristics of significant GHS response and controlled drug release.

关键词

电喷氧化还原响应性核壳结构微粒

Keywords

ElectrosprayRedox responsiveCore-shell structure microparticle

references

Y Wang , Y Zhang , B Wang , Y Cao , Q Yu , T Yin . J Nanopart Res , 2013 . 15 ( 6 ): 1 - 12.

V Torchilin . Eur J Pharm Biopharm , 2009 . 71 ( 3 ): 431 - 444 . DOI:10.1016/j.ejpb.2008.09.026http://doi.org/10.1016/j.ejpb.2008.09.026.

J Chen , H Dai , H Lin , K Tu , H Wang , L Q Wang . Colloid Surf B , 2016 . 141 278 - 283 . DOI:10.1016/j.colsurfb.2016.01.051http://doi.org/10.1016/j.colsurfb.2016.01.051.

Z Liu , Y Jiao , Y Wang , C Zhou , Z Zhang . Adv Drug Delivery Rev , 2008 . 60 ( 15 ): 1650 - 1662 . DOI:10.1016/j.addr.2008.09.001http://doi.org/10.1016/j.addr.2008.09.001.

Y Zhu , J Shi , W Shen , X Dong , J Feng , M Ruan , Y Li . Angew Chem , 2005 . 117 ( 32 ): 5213 - 5217 . DOI:10.1002/(ISSN)1521-3757http://doi.org/10.1002/(ISSN)1521-3757.

X Huang , C S Brazel . J Control Release , 2001 . 73 ( 2 ): 121 - 136.

P Carmeliet , R K Jain . Nature , 2000 . 407 ( 6801 ): 249 - 257 . DOI:10.1038/35025220http://doi.org/10.1038/35025220.

L Y Tang , Y C Wang , Y Li , J Z Du , J Wang . Bioconjugate Chem , 2009 . 20 ( 6 ): 1095 - 1099 . DOI:10.1021/bc900144mhttp://doi.org/10.1021/bc900144m.

D Maciel , P Figueira , S Xiao , D Hu , J Rodrigues , H Tomas , Y Li . Biomacromolecules , 2013 . 14 ( 9 ): 3140 - 3146 . DOI:10.1021/bm400768mhttp://doi.org/10.1021/bm400768m.

A Russo , W DeGraff , N Friedman , J B Mitchell . Cancer Res , 1986 . 46 ( 6 ): 2845 - 2848.

Y Cao , J Zhao , Y Zhang , J Liu , J Liu , A Dong , L Deng . RSC Adv , 2015 . 5 ( 36 ): 28060 - 28069 . DOI:10.1039/C5RA01833Ahttp://doi.org/10.1039/C5RA01833A.

Y Wang , G Wu , X Li , J Chen , Y Wang , J Ma . J Mater Chem , 2012 . 22 ( 48 ): 25217 - 25226 . DOI:10.1039/c2jm35186bhttp://doi.org/10.1039/c2jm35186b.

R Cheng , F Feng , F Meng , C Deng , J Feijen , Z Zhong . J Control Release , 2011 . 152 ( 1 ): 2 - 12 . DOI:10.1016/j.jconrel.2011.01.030http://doi.org/10.1016/j.jconrel.2011.01.030.

M Huo , J Yuan , L Tao , Y Wei . Polym Chem , 2014 . 5 ( 5 ): 1519 - 1528 . DOI:10.1039/C3PY01192Ehttp://doi.org/10.1039/C3PY01192E.

Y C Wang , F Wang , T M Sun , J Wang . Bioconjugate Chem , 2011 . 22 ( 10 ): 1939 - 1945 . DOI:10.1021/bc200139nhttp://doi.org/10.1021/bc200139n.

H Sun , B Guo , X Li , R Cheng , F Meng , H Liu , Z Zhong . Biomacromolecules , 2010 . 11 ( 4 ): 848 - 854 . DOI:10.1021/bm1001069http://doi.org/10.1021/bm1001069.

J Wang , W Mao , L L Lock , J Tang , M Sui , W Sun , H Cui , D Xu , Y Shen . ACS Nano , 2015 . 9 ( 7 ): 7195 - 7206 . DOI:10.1021/acsnano.5b02017http://doi.org/10.1021/acsnano.5b02017.

L Cao , J Luo , K Tu , L Q Wang , H Jiang . Colloids Surf, B , 2014 . 115 212 - 218 . DOI:10.1016/j.colsurfb.2013.11.046http://doi.org/10.1016/j.colsurfb.2013.11.046.

A Jaworek . Powder Technol , 2007 . 176 ( 1 ): 18 - 35 . DOI:10.1016/j.powtec.2007.01.035http://doi.org/10.1016/j.powtec.2007.01.035.

A Jaworek , A T Sobczyk . J Electrost , 2008 . 66 ( 3 ): 197 - 219.

Y Xu , M Skotak , M Hanna . J Microencapsul , 2006 . 23 ( 1 ): 69 - 78 . DOI:10.1080/02652040500435048http://doi.org/10.1080/02652040500435048.

S Zhang , K Kawakami , M Yamamoto , Y Masaoka , M Kataoka , S Yamashita , S Sakuma . Mol Pharmaceutics , 2011 . 8 ( 3 ): 807 - 813 . DOI:10.1021/mp100401dhttp://doi.org/10.1021/mp100401d.

I G Loscertales , A Barrero , I Guerrero , R Cortijo , M Marquez , A M Ganan-Calvo . Science , 2002 . 295 ( 5560 ): 1695 - 1698 . DOI:10.1126/science.1067595http://doi.org/10.1126/science.1067595.

Y H Lee , F Mei , M Y Bai , S Zhao , D R Chen . J Control Release , 2010 . 145 ( 1 ): 58 - 65 . DOI:10.1016/j.jconrel.2010.03.014http://doi.org/10.1016/j.jconrel.2010.03.014.

S D Perrault , C Walkey , T Jennings , H C Fischer , W C W Chan . Nano Lett , 2009 . 9 ( 5 ): 1909 - 1915 . DOI:10.1021/nl900031yhttp://doi.org/10.1021/nl900031y.

S R van Tomme , W E Hennink . Expert Rev Med Devices , 2007 . 4 ( 2 ): 147 - 164 . DOI:10.1586/17434440.4.2.147http://doi.org/10.1586/17434440.4.2.147.

H Jiang , Y Hu , Y Li , P Zhao , K Zhu , W Chen . J Control Release , 2005 . 108 ( 2 ): 237 - 243.

S L Shenoy , W D Bates , H L Frisch , G E Wnek . Polymer , 2005 . 46 ( 10 ): 3372 - 3384 . DOI:10.1016/j.polymer.2005.03.011http://doi.org/10.1016/j.polymer.2005.03.011.

A M Ganan-Calvo , J Davila , A Barrero . J Aerosol Sci , 1997 . 28 ( 2 ): 249 - 275 . DOI:10.1016/S0021-8502(96)00433-8http://doi.org/10.1016/S0021-8502(96)00433-8.

J Yao , L K Lim , J Xie , J Hua , C H Wang . J Aerosol Sci , 2008 . 39 ( 11 ): 987 - 1002 . DOI:10.1016/j.jaerosci.2008.07.003http://doi.org/10.1016/j.jaerosci.2008.07.003.

更多指标>

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Fabrication of MMP-2 Rresponsive Nanoparticles by Coaxial Electrospray-template Removal

Related Author

No data

Related Institution

MOE Key Laboratory of Macromolecular Synthesis and Functionalization

Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University

Postal code：100190
Tel：010-62588927 Email：gfzxb@iccas.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05002797号-8 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰