Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles

Song Tao; Xi Yue-jing; Du Jian-zhong

doi:10.11777/j.issn1000-3304.2018.17229

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Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles

Research Article | 更新时间：2021-01-26

- Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles
  增强出版
- Acta Polymerica Sinica Issue 1, Pages: 119-128(2018)
- 作者机构：
  
  先进土木工程材料教育部重点实验室同济大学材料科学与工程学院上海 201804
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2018.17229
  CLC：
- Published：20 January 2018，
  
  Received：17 August 2017，
  
  Revised：13 September 2017，
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Song Tao, Xi Yue-jing, Du Jian-zhong. Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles. [J]. Acta Polymerica Sinica (1):119-128(2018)
DOI：

Song Tao, Xi Yue-jing, Du Jian-zhong. Antibacterial Hydrogels Incoporated with Poly(glutamic acid)-based Vesicles. [J]. Acta Polymerica Sinica (1):119-128(2018) DOI： 10.11777/j.issn1000-3304.2018.17229.

摘要

通过开环聚合（ROP）合成了两亲嵌段共聚物聚己内酯-聚谷氨酸（PCL-

-PGA），并将其自组装成囊泡，然后利用聚谷氨酸的羧基原位沉积纳米银颗粒，得到了抗菌囊泡.最后，将抗菌囊泡与普朗尼克F127基体混合，制备了抗菌水凝胶.实验结果表明抗菌囊泡对典型革兰氏阴性菌如大肠杆菌和典型革兰氏阳性菌如金黄色葡萄球菌的MIC

（抑制90%菌株生长的最低浓度）分别为10和20 μg mL

-1

.平板菌落计数法表明抗菌水凝胶对大肠杆菌和金黄色葡萄球菌的MIC

（抑制50%菌株生长的最低浓度）为30 μg mL

-1

，MIC

为60 μg mL

-1

.外敷法抗菌实验也证明了水凝胶具有优异的抗菌效果，其对大肠杆菌和金黄色葡萄球菌的MIC

为7.5 μg mL

-1

，MBC（最小杀菌浓度）为30 μg mL

-1

Abstract

We designed a hydrogel with durable and excellent antibacterial activity. Amphiphilic block copolymer

polycaprolactone-block-poly(glutamic acid) (PCL-

-PGA)

was first synthesized by ring-opening polymerization with amine-terminated polycaprolactone (PCL-NH

) as the macro-initiator and Bz-Glu-NCA as the monomer. This copolymer self-assembled into vesicles in solvent mixture of DMF/water with a critical vesiculation concentration of 5.27 μg mL

-1

. Antibacterial Ag-decorated vesicles were then prepared by

in situ

deposition of silver nanoparticles in PGA coronas of the vesicles as a result of the electrostatic interaction between carboxyl groups and Ag

H-NMR analysis revealed the composition of PCL

-PGA

block copolymer. Dynamic light scattering (DLS) study revealed an intensity-averaged diameter of 215 nm for the vesicles with a polydispersity of 0.029. Transmission electron microscopy (TEM) confirmed a hollow structure of the vesicles with a number-averaged diameter of 270 nm

and the presence of sliver nanoparticles on the vesicles. Atomic force microscopy (AFM) revealed the collapsed surface morphology of vesicles with a width to height ratio of 18

confirming further the hollow structure of the vesicles. Meanwhile

Ag-decorated vesicles were incoporated into Pluronic F127 hydrogels to afford antibacterial hydrogels

showing good degradability at different pH. MIC

(minimum concentration of inhibiting 90% of bacterium) of the antibacterial vesicles was 10 μg mL

-1

against Gram-negative bacterium

E. coli

and it was 20 μg mL

-1

against Gram-positive bacterium

S. aureus

and measured by spread plate method

which afford the hydrogels excellent antibacterial property. Oxford cup tests confirmed that MIC

of the antibacterial hydrogel against both

E. coli

and

S. aureus

is 7.5 μg mL

-1

and MBC (minimum bactericidal concentration) against both of them is 30 μg mL

-1

. The

in vitro

degradation tests confirmed that the vesicle was degraded in the presence of lipase. Overall

we provide a new method for preparation of antibacterial hydrogels

which may have promising biomedical applications requiring a long-acting antibacterial therapy such as inflammation triggered by bacteria infection

wound healing after surgery and sterilization for the implant devices

etc

关键词

囊泡聚氨基酸抗菌水凝胶自组装

Keywords

VesiclesPoly(amino acid)sAntibacterial propertyHydrogelsSelf-assembly

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State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences, Nankai University

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