Water-soluble Conjugated Polymers for Biosensor Applications

En-dong Zhang; Li-bing Liu; Feng-ting Lv; Shu Wang

doi:10.11777/j.issn1000-3304.2018.17269

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Water-soluble Conjugated Polymers for Biosensor Applications

Feature Articles | 更新时间：2021-01-26

- Water-soluble Conjugated Polymers for Biosensor Applications
- Acta Polymerica Sinica Issue 2, Pages: 186-197(2018)
- 作者机构：
  
  1.中国科学院化学研究所北京 100190
  2.中国科学院大学北京 100049
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2018.17269
  CLC：
- Published：20 February 2018，
  
  Received：18 September 2017，
  
  Revised：2 November 2017，
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En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications. [J]. Acta Polymerica Sinica (2):186-197(2018)
DOI：

En-dong Zhang, Li-bing Liu, Feng-ting Lv, Shu Wang. Water-soluble Conjugated Polymers for Biosensor Applications. [J]. Acta Polymerica Sinica (2):186-197(2018) DOI： 10.11777/j.issn1000-3304.2018.17269.

摘要

水溶性共轭聚合物在化学、医学、生命科学以及材料科学等领域中备受研究者们的关注，利用其独特的光化学和光物理性质，研究者们开展了一系列创新性研究并取得了重大的研究进展，进而拓展了聚合物的应用范围.共轭聚合物是一种由多个重复发光单元通过彼此间共轭而形成的高分子化合物，通过对其结构的精准调控，可以获得具有不同性能的功能性分子.其中，通过改变其主链结构，可以获得具有不同吸收和发射波长的荧光探针；通过对侧链结构修饰以水溶性基团和/或选择性识别分子，可以实现与特定靶标间的静电作用或者特异性结合，进而实现选择性识别的目的.本文综述了近年来水溶性共轭聚合物在生物传感中的应用，主要介绍了水溶性共轭聚合物在DNA检测、蛋白质检测、细胞和细菌的检测与区分以及细胞成像等方面的研究进展.

Abstract

In recent years

conjugated polymers (CPs) with characteristic and excellent photochemical and photophysical properties have become particularly popular in scientific research and are widely used for biological sensing. Conjugated polymers are characterized by a delocalized electronic structure along their backbones and exhibit electronic coupling between each optoelectronic segments. Owing to unique delocalized

-conjugated structure

CPs are endowed with distinguished optical properties

such as light-harvesting and amplification capability and high energy transfer efficiency. Water-soluble conjugated polymers (WSCPs) introduced with charged groups or selectively-recognition elements on the side chains can specifically diagnose through electrostatic interaction or specific binding with targets. In addition

WSCPs with diverse absorption and emission characteristics have been designed and synthesized by tuning the constitution and conformation of the backbones. In contrast with fluorescence proteins

small organic molecules and quantum dots

CPs with greatly unique properties have greater advantages that directed to sensing and imaging in biochemical and biomedical fields. This review article is aimed to summarize recent research progresses on applications of WSCPs as biosensors for the detection of a series of biological targets. Firstly

according to electrostatics interaction of CPs and targeted DNA

different novel strategies are used for detection of DNA mutations (section 2). Meanwhile

CPs modified with recognition moieties can be utilized to detect specific proteins (section 3). In addition

based on different interaction modes between CPs and cells and pathogens

identification and discrimination of microbial pathogens and cells are realized (section 4). CPs can also be used for cell imaging due to their advantageous optical properties (section 5). The structures

characteristics and advantages of CPs

as well as various nove lidentifications and detections strategies are also discussed. Through a variety of design and modification

novel CPs can be obtained and used for identification and detection

which is complementary to traditional CPs in biosensor and widens the range of biological applications. It is worth mentioning that

with the development of inter-discipline

more attention should be paid to the combination of new methods and strategies for exploiting functions of CPs in biological sensing.

关键词

水溶性共轭聚合物生物传感信号放大识别检测生物成像

Keywords

Conjugated polymerBiosensorSignal amplificationIdentification and determinationCell imaging

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