Preparation and Photo-thermal Properties of Thermo-sensitive Injectable Hydrogels Based on Chitosan and Au Nanoparticles

Jin-feng Zeng; Wen-di Yang; Dong-jian Shi; Xiao-jie Li; Ming-qing Chen

doi:10.11777/j.issn1000-3304.2018.18048

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Preparation and Photo-thermal Properties of Thermo-sensitive Injectable Hydrogels Based on Chitosan and Au Nanoparticles

Research Article | 更新时间：2021-01-26

- Preparation and Photo-thermal Properties of Thermo-sensitive Injectable Hydrogels Based on Chitosan and Au Nanoparticles
- Acta Polymerica Sinica Vol. 0, Issue 10, Pages: 1297-1306(2018)
- 作者机构：
  
  合成与生物胶体教育部重点实验室江南大学化学与材料工程学院　无锡 214122
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2018.18048
  CLC：
- Published：2018-10，
  
  Received：5 February 2018，
  
  Revised：28 March 2018，
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Jin-feng Zeng, Wen-di Yang, Dong-jian Shi, Xiao-jie Li, Ming-qing Chen. Preparation and Photo-thermal Properties of Thermo-sensitive Injectable Hydrogels Based on Chitosan and Au Nanoparticles. [J]. Acta Polymerica Sinica 0(10):1297-1306(2018)
DOI：

Jin-feng Zeng, Wen-di Yang, Dong-jian Shi, Xiao-jie Li, Ming-qing Chen. Preparation and Photo-thermal Properties of Thermo-sensitive Injectable Hydrogels Based on Chitosan and Au Nanoparticles. [J]. Acta Polymerica Sinica 0(10):1297-1306(2018) DOI： 10.11777/j.issn1000-3304.2018.18048.

摘要

为提高对恶性肿瘤的治疗效率，制备了具有光热性能的壳聚糖/纳米金可注射性水凝胶用于肿瘤的多次光热治疗. 选用生物基大分子壳聚糖(CS)原位还原制备了壳聚糖-纳米金(CS-Au NPs)复合物. 再加入

-甘油磷酸钠(

-GP)制备了相转变温度接近人体体温的温敏性水凝胶(CGP/Au NPs). 实验结果表明，CGP/Au NPs在室温下为溶胶，具有可注射性；达到人体体温后，快速形成凝胶. 由于Au NPs的表面等离子体共振效应，基于壳聚糖/纳米金的复合凝胶在激光照射下具有优异的光热转换性能，最高温度可达55 °C，而且在多次激光照射后仍可以保持较好的光热转换能力. Au NPs可在壳聚糖水凝胶的帮助下长时间固定于病灶，用于肿瘤的多次光热治疗. 另外，CGP/Au NPs复合水凝胶具有良好的稳定性，Au NPs在凝胶内部分布均匀一致，可有效避免Au NPs聚集而产生局部高温或从凝胶内部泄漏而影响光热治疗的效果. 同时，CGP/Au NPs具有优异的生物相容性和生物可降解性能. 因此，基于壳聚糖与Au NPs的可注射性水凝胶，有望实现肿瘤的“一次注射，多次光热治疗”的目的.

Abstract

Multiple injections of drugs are generally needed to continuously and effectively kill pathological cells during tumor therapeutic. However

this strategy might induce some side effects. Gold nanoparticles are one kind of photothermal agents that can convert the absorbed light energy into heat energy. This conversion could be used to photo-thermal therapy (PTT)

which has property of multiple treatment for killing the tumor cells effectively and completely without other effects. Herein

this study aims to prepare injectable hydrogels with PTT property based on chitosan (CS)

-glycerophosphate (

-GP) and gold nanoparticles (Au NPs) for improving efficiency of anti-tumour. Firstly

CS bio-polymer stabilized Au NPs (CS-Au NPs) were directly reduced

in situ

by CS. Size of the CS-Au NPs could be controlled at 15 nm. Then

-glycerophosphate (

-GP) was added into the CS-Au NPs. The mixture of CS-Au NPs and

-GP was sol state at room temperature

which could be injected into the tumor site. And then

at physiological temprature (37 °C)

this sol could convert to gel (CGP/Au NPs)

via

the interactions between CS and

-GP

indicating that the (CGP/Au NPs) gel had the temperature sensitivity. Due to the surface plasmon resonance effect (SPR) of Au NPs

the (CGP/Au NPs) hydrogels showed an excellent photo-thermal property. The temperature of the (CGP/Au NPs) gel rised to above 45 °C and further to approximately 55 °C under Laser irradiation

with increasing amout of Au NPs. Moreover

the photo-thermal ability could be kept for several cycles with repeated laser irradiation. Because of easily fixing CGP hydrogels into biological tissue

Au NPs could be immobilized on the tumor site for a long time for multiple PTT. Thus

the strategies of muiliple drug-injection and blood circulation could be changed with the help of CGP hydrogels. Additionally

Au NPs were distributed evenly in the CGP hydrogels and the (CGP/Au NPs) hydrogels had good stability. Cell viability showed that the gels had excellent biocompatibility to the normal cells

and could kill remarkably the cancer cells with laser irradiation. Meanwhile

the prepared thermo-sensitive hydrogels had good biodegradability. Due to these characteristics

this (CGP/Au NPs) hydrogel might have the potential application to achieve " one time injection

multiple treatments” for tumor therapy.

关键词

温敏性水凝胶光热治疗纳米金

Keywords

Thermo-sensitive hydrogelsPhoto-thermal therapyGold nanoparticles

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