Self-assembly of Protein-Polymer Conjugate on Gold Surface Studied by Surface Plasmon Resonance Spectroscopy

Jin Li; Wen-wen Wang; Li-qiang Chu

doi:10.11777/j.issn1000-3304.2018.18063

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Self-assembly of Protein-Polymer Conjugate on Gold Surface Studied by Surface Plasmon Resonance Spectroscopy

Research Article | 更新时间：2021-01-26

- Self-assembly of Protein-Polymer Conjugate on Gold Surface Studied by Surface Plasmon Resonance Spectroscopy
- Acta Polymerica Sinica Vol. 0, Issue 10, Pages: 1328-1335(2018)
- 作者机构：
  
  天津科技大学化工与材料学院　天津 300457
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2018.18063
  CLC：
- Published：2018-10，
  
  Received：13 February 2018，
  
  Revised：29 March 2018，
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Jin Li, Wen-wen Wang, Li-qiang Chu. Self-assembly of Protein-Polymer Conjugate on Gold Surface Studied by Surface Plasmon Resonance Spectroscopy. [J]. Acta Polymerica Sinica 0(10):1328-1335(2018)
DOI：

Jin Li, Wen-wen Wang, Li-qiang Chu. Self-assembly of Protein-Polymer Conjugate on Gold Surface Studied by Surface Plasmon Resonance Spectroscopy. [J]. Acta Polymerica Sinica 0(10):1328-1335(2018) DOI： 10.11777/j.issn1000-3304.2018.18063.

摘要

研究了蛋白质-高分子结合体在金片表面的自组装行为. 首先制备了一种引发剂2-溴-2-甲基丙酸-2-氨氧基乙酯(ABM)，该引发剂可以与5′-磷酸吡哆醛(PLP)活化的牛血清白蛋白(BSA)的氮端相连，进而得到大分子引发剂BSA-Br. 最后以甲基丙烯酸寡聚乙二醇酯(OEGMA)为单体，利用原子转移自由基聚合法(ATRP)制备BSA-POEGMA结合体. 利用红外光谱(FTIR)、紫外可见分光光度计(UV-Vis)、基质辅助激光解析串联飞行时间质谱仪(MALDI-TOF-MS)对各步产物进行表征. 利用表面等离子体共振光谱(SPR)研究BSA-POEGMA结合体在金片表面的自组装行为，以及金片表面吸附的BSA-POEGMA结合体对其他蛋白质包括溶菌酶(Lys)和纤维蛋白原(Fib)的吸附效果影响.

Abstract

Recently

protein-polymer conjugates

which combine protein molecules with one or more specific polymer chains at certain position

have attracted a great deal of attention due to their unique properties of both the proteins and the polymers. Therefore

the objective of this work is to study the self-assembly behavior of protein-polymer conjugates on a planar gold substrate

in which bovine serum albumin (BSA) is employed as the anchoring point for self-assembly process. We first prepare an initiator

2-bromo-2-methylpropionic acid-2-aminooxy ethyl ester (ABM)

which can be linked to the N-terminal of BSA modified by pyridoxal-5-phosphate (PLP)

giving rise to a macroinitiator (

i.e.

BSA-Br). Then BSA-POEGMA conjugate is obtained by atom transfer radical polymerization (ATRP) using oligo(ethylene glycol) methacrylate (OEGMA) as monomer and the BSA-Br as macroinitiator

respectively. All products are characterized by Fourier-transform infrared spectroscopy (FTIR)

ultraviolet-visible spectroscopy (UV-Vis) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). FTIR and MALDI-TOF-MS results indicate that one POEGMA chain with a polymerization degree of 218 is coupled to the N-terminal of BSA. The adsorption behaviors of both the BSA and the BSA-POEGMA conjugate on gold surfaces are studied by using surface plasmon resonance spectroscopy (SPR). The data suggest that the BSA-POEGMA conjugate could be adsorbed to the gold substrate at the same way as the pure BSA molecule as expected

while the hydrophilic POEGMA chain is directed to the opposite of gold surface. At last

the adsorption behaviors of two model proteins (

i.e.

lysozyme

Lys and fibrinogen

Fib) on the BSA-POEGMA conjugate are also investigated. SPR data indicate that there is a decrease in Lys adsorption on the BSA-POEGMA compared to pure BSA layer. In contrast

Fib adsorption on the BSA-POEGMA conjugate increase slightly in comparison with that on pure BSA. The results clearly show the influence of POEGMA chain on the surface property of BSA molecule

which may be attributed to the water layer associated with the POEGMA chains.

关键词

蛋白质-高分子结合体牛血清白蛋白聚甲基丙烯酸寡聚乙二醇酯表面等离子体共振光谱

Keywords

Protein-polymer conjugateBovine serum albuminPoly(oligo(ethylene glycol) methacrylate)Surface plasmon resonance

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