Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells

Jian-xu Zhang; Ting-ting Sun; Zhi-gang Xie

doi:10.11777/j.issn1000-3304.2019.19001

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Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells

Research Article | 更新时间：2021-01-26

- Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells
  增强出版
- Acta Polymerica Sinica Vol. 50, Issue 6, Pages: 633-641(2019)
- 作者机构：
  
  高分子物理与化学国家重点实验室中国科学院长春应用化学研究所　长春 130022
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2019.19001
  CLC：
- Published：2019-6，
  
  Published Online：28 March 2019，
  
  Received：3 January 2019，
  
  Revised：12 February 2019，
扫描看全文
Jian-xu Zhang, Ting-ting Sun, Zhi-gang Xie. Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells. [J]. Acta Polymerica Sinica 50(6):633-641(2019)
DOI：

Jian-xu Zhang, Ting-ting Sun, Zhi-gang Xie. Dye-protein Hybrid Nanoparticles for Photothermal Therapy of Tumor Cells. [J]. Acta Polymerica Sinica 50(6):633-641(2019) DOI： 10.11777/j.issn1000-3304.2019.19001.

摘要

为了解决花菁类(Cypate)近红外染料作为光热试剂的一些固有缺点，例如疏水性、毒性、不稳定性等，以牛血清白蛋白和Cypate染料作为原料，在水热条件下制备出尺寸较小的稳定杂化纳米粒. 纳米结构显著改善了Cypate染料的水溶性，也提升了染料在水相溶液中的稳定性. 与小分子染料相比，染料杂化蛋白纳米粒具有良好的生物相容性和光稳定性. 此外，蛋白纳米粒具有优于小分子染料的光热转换能力，并展现了良好的光热细胞杀伤能力.

Abstract

To make up for the inherent drawbacks (

e.g

. hydrophobicity

toxicity

and instability) of near-infrared Cypate dyes

as photothermal agents

stable hybrid nanoparticles (CBNPs) with small size were prepared from bovine serum albumin (BSA) and Cypate dyes under hydrothermal conditions. Free Cypate exhibited strong fluorescence whereas fluorescence quenching in CBNPs caused a sharp decrease in emission peak intensity

so the dye molecules were successfully assembled into protein nanoparticles. As-prepared CBNPs were characterized with dynamic light scattering (DLS) and transmission electron microscopy (TEM). The hydrodynamic diameter of CBNPs was around 25 – 40 nm with a low polydispersity index of 0.2

slightly higher than the measured values from TEM observation. BSA encapsulation could significantly improve the water solubility of Cypate dye and the stability of dye aqueous solutions. Besides

these nanoparticles showed good colloidal stability and biocompatibility. Photothermal experiments suggested that the protein nanoparticles had good photothermal performance and were able to generate enough heat under near-infrared laser irradiation. The photothermal conversion efficiency (

) of CBNPs under 808 nm laser irradiation reached up to 50%

which made them outperform the small molecules of neat Cypate dyes in terms of their photothermal conversion capabilities. Confocal laser scanning microscopy further revealed that the protein nanoparticles could be efficiently internalized by cancer cells in a time-dependent manner

which is very important for their therapeutic functions. Photothermal treatment toward human cervical carcinoma (HeLa) cells and human liver hepatocellular carcinoma (HepG2) cells under laser irradiation was extamined by MTT method

in which the protein nanoparticles showed an effective inhibition effect on tumor cell proliferation at the cellular level. Live/dead cell staining experiments conducted on HeLa cells also showed the same results intuitively. The dye-protein hybrid nanoparticles developed in this study as a novel nano-agent possess promising prospects in the field of tumor photothermal therapy.

关键词

蛋白纳米粒花菁类染料超分子组装光热治疗

Keywords

Protein nanoparticlesCypate dyesSupramolecular assemblyPhotothermal therapy

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