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Construction and Application of Size-controllable Spherical DNA Nanoparticles
增强出版- ACTA POLYMERICA SINICA Vol. 54, Issue 3, Pages: 336-345(2023)
- 作者机构：
1.中国人民大学化学系 北京 100872
2.中国科学院化学研究所 北京 100190
3.中国科学院大学 北京 100049
- 作者简介：
Zhi-yong Yu, E-mail: Yuzhiyong@ruc.edu.cn
Li-jin Xu, E-mail: 20050062@ruc.edu.cn
Yuan-chen Dong, E-mail: dongyc@iccas.ac.cn
- 基金信息：
DOI：10.11777/j.issn1000-3304.2022.22323
CLC：Published：20 March 2023，
Published Online：16 November 2022，
Received：23 September 2022，
Accepted：21 October 2022
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成佳峰,袁伟,丁雨樵等.尺寸可控的球型DNA纳米颗粒构筑策略及其应用[J].高分子学报,2023,54(03):336-345.
Cheng Jia-feng,Yuan Wei,Ding Yu-qiao,et al.Construction and Application of Size-controllable Spherical DNA Nanoparticles[J].ACTA POLYMERICA SINICA,2023,54(03):336-345.
成佳峰,袁伟,丁雨樵等.尺寸可控的球型DNA纳米颗粒构筑策略及其应用[J].高分子学报,2023,54(03):336-345. DOI： 10.11777/j.issn1000-3304.2022.22323.
Cheng Jia-feng,Yuan Wei,Ding Yu-qiao,et al.Construction and Application of Size-controllable Spherical DNA Nanoparticles[J].ACTA POLYMERICA SINICA,2023,54(03):336-345. DOI： 10.11777/j.issn1000-3304.2022.22323.
摘要
为探究球型DNA纳米颗粒的尺寸对细胞摄取的影响，提出了一种新型构筑策略，制备了一系列尺寸精准可控的球型DNA纳米颗粒，并进一步揭示了球型DNA纳米颗粒的尺寸与MCF-7细胞内化效率的关系；此外，该策略还可以与框架诱导策略相结合，构筑尺寸可控的磷脂囊泡. 该结果为构建新型高效的DNA纳米颗粒载药系统提供了理论参考，为特定尺寸的药物载体设计和开发拓展了思路.
Abstract
During last decades
spherical DNA nanoparticles have been demonstrated as a powerful tool for delivering nucleic acids
small molecular drugs and protein
etc
. While the importance of the sizes of such nanoparticles has been recognized in cell uptake efficiency
the investigation in detailed mechanism is still limited owing to the lack of tools to prepare size controllable nanoparticles. Herein
we have developed a strategy to construct spherical DNA nanoparticles (AuNP-G
x
) with precise and controllable sizes
through the assembly of DNA modified gold nanoparticles and rigid DNA dendrimers (Y
1
and Y
2
) in a layer-by-layer manner. The rigid DNA dendrimers were firstly assembled through three different single strands DNA . Each dendrimer structure (Y
1
and Y
2
) was designed with the sticky-end segments which can further hybridize with the other Y. Therefore
through stepwise incubation with the gold particle core
different particles could be easily prepared through such only two components. The dynamic light scattering (DLS)
agarose gel electrophoresis and transmission electron microscopy (TEM) showed that DNA dendrimers have been assembled around gold nanoparticles layer-by-layer
which indicated the successful preparation of AuNP-G
x
with precise and controllable sizes. The prepared AuNP-G
x
nucleic acid nanoparticles were successfully used to reveal the size effect of nucleic acid nanoparticle on the uptake efficiency of MCF-7 cells. From the experimental results
it could be observed that AuNP-G
1
and AuNP-G
0
performed the highest internalization efficiency for MCF-7 cells
while the internalization efficiency of the nanoparticles decreased gradually with the increase of the size. A size-controllable phospholipid vesicle with drug encapsulation and delivery potential was also constructed by using AuNP-G
x
of different sizes as the frames and using the Frame-Guided Assembly strategy. Besides
the phospholipid vesicles were easily ingested by MCF-7 cells and showed good biocompatibility. This strategy provides a new tool for the design and development of drug carriers with specific dimensions
and a new theoretical support for the construction of novel and efficient DNA nanoparticle drug-loading systems.
关键词
尺寸可控球型DNA纳米颗粒DNA自组装框架诱导组装
Keywords
Controllable sizeSpherical DNA nanoparticlesDNA self-assemblyFrame-guided assembly
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袁伟, 董原辰. 框架诱导组装策略研究进展. 高分子学报, 2022, 53, 1204-1216. doi:10.11777/j.issn1000-3304.2022.22154http://dx.doi.org/10.11777/j.issn1000-3304.2022.22154
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