Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning

Jing Chen; Chang Liu; Bing Yu; Hai-lin Cong

doi:10.11777/j.issn1000-3304.2023.23114

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Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning

Research Article | 更新时间：2023-09-25

- Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning
- ACTA POLYMERICA SINICA Vol. 54, Issue 10, Pages: 1632-1640(2023)
- 作者机构：
  
  1.青岛大学化学化工学院青岛 266071
  2.山东理工大学材料科学与工程学院淄博 255000
- 作者简介：
  
  Bing Yu, E-mail: yubing198@qdu.edu.cn
  Hai-lin Cong, E-mail: conghailin@sdut.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2023.23114
  CLC：
- Published：20 October 2023，
  
  Published Online：15 August 2023，
  
  Received：23 May 2023，
  
  Accepted：28 June 2023
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陈静,刘畅,于冰等.基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为[J].高分子学报,2023,54(10):1632-1640.

Chen Jing,Liu Chang,Yu Bing,et al.Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning[J].ACTA POLYMERICA SINICA,2023,54(10):1632-1640.
陈静,刘畅,于冰等.基于静电纺丝的类海绵体材料用于溶菌酶的吸附行为[J].高分子学报,2023,54(10):1632-1640. DOI： 10.11777/j.issn1000-3304.2023.23114.

Chen Jing,Liu Chang,Yu Bing,et al.Adsorption Behavior of Lysozyme by Sponge-like Materials Based on Electrospinning[J].ACTA POLYMERICA SINICA,2023,54(10):1632-1640. DOI： 10.11777/j.issn1000-3304.2023.23114.

摘要

用SiO

纳米纤维作为三维结构刚性支撑框架，以聚乙烯醇(PVA)/丁烷四羧酸(BTCA)的酯化交联反应物为功能性有机黏合剂，制备了一种高度羧基化的海绵体整体吸附材料(PVA/BTCA多孔材料)，得到的PVA/BTCA材料具有多孔结构. 作为一种吸附材料，通过水下循环压缩性能测试证明PVA/BTCA材料在水下拥有良好的结构稳定性. PVA/BTCA多孔材料的高度羧酸化和高孔隙率为蛋白吸附提供了基础条件，可以选择性吸附带正电荷的蛋白. 以溶菌酶为主要模型蛋白，PVA/BTCA多孔材料在pH=7时对溶菌酶的最大吸附量为1342.3 mg/g，PVA/BTCA多孔材料对溶菌酶的吸附的等温吸附模型与Freundlich吸附模型接近一致. 优秀的机械稳定性和化学结构稳定性赋予PVA/BTCA多孔材料可重复使用性，这项工作有望为蛋白分离方向提供一种新的分离材料.

Abstract

A highly carboxylated sponge monolithic adsorbent material (PVA/BTCA porous material) was prepared using SiO

nanofibers as a three-dimensional structural rigid support framework and an esterified cross-linking reactant of poly(vinyl alcohol) (PVA)/butane tetracarboxylic acid (BTCA) as a functional organic binder. As a kind of adsorption material

it is proven that PVA/BTCA material has good structural stability under water by underwater cyclic compression test. The highly carboxylated and high porosity of the PVA/BTCA porous material provided the basis for protein adsorption

which could selectively adsorb positively charged proteins. Using lysozyme as the main model protein

the maximum adsorption of PVA/BTCA porous material for lysozyme at pH=7 was 1342.3 mg/g. The isothermal adsorption model of PVA/BTCA porous material for the adsorption of lysozyme is in close agreement with the Freundlich adsorption model. The excellent mechanical stability and chemical structural stability confer reusability to the PVA/BTCA porous material. This work is expected to provide a new separation material for protein separation.

关键词

溶菌酶三维材料吸附聚乙烯醇

Keywords

LysozymeThree-dimensional materialAdsorptionPoly(vinyl alcohol)

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University of Science and Technology of China

CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences

Key Laboratory for Polymeric Composite & Functional Materials of Ministry of Education, School of Chemistry, Sun Yat-Sen University

School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Key Laboratory of Multifunctional Nanomaterials and Smart Systems, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences

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