Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures

Xue-hui Zhang; Yan-qin Wang; Qiang Zheng

doi:10.11777/j.issn1000-3304.2023.23275

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Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures

Research Article | 更新时间：2024-08-14

- Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures
- Acta Polymerica Sinica Vol. 55, Issue 8, Pages: 1069-1080(2024)
- 作者机构：
  
  1.太原理工大学，材料科学与工程学院，浙江大学高分子科学与工程学系杭州 310027
  2.(太原理工大学，生物医学工程学院太原 030024) (，浙江大学高分子科学与工程学系杭州 310027)
  3.(太原理工大学，山西浙大新材料与化工研究院太原 030001) (，浙江大学高分子科学与工程学系杭州 310027)
  4.太原理工大学，高分子合成与功能构造教育部重点实验室，浙江大学高分子科学与工程学系杭州 310027
- 作者简介：
  
  Yan-qin Wang, E-mail: wangyanqin@tyut.edu.cn
  Qiang Zheng, E-mail: zhengqiang@zju.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2023.23275
  CLC：
- Published：20 August 2024，
  
  Published Online：09 May 2024，
  
  Received：30 November 2023，
  
  Accepted：22 March 2024
- 稿件说明：
移动端阅览
张雪慧, 王艳芹, 郑强. 仿生各向异性结构复合水凝胶及其流变学行为. 高分子学报, 2024, 55(8), 1069-1080

Zhang, X. H.; Wang, Y. Q.; Zheng, Q. Rheological behavior of biomimetic composite hydrogels with anisotropic structures. Acta Polymerica Sinica, 2024, 55(8), 1069-1080
张雪慧, 王艳芹, 郑强. 仿生各向异性结构复合水凝胶及其流变学行为. 高分子学报, 2024, 55(8), 1069-1080 DOI： 10.11777/j.issn1000-3304.2023.23275.

Zhang, X. H.; Wang, Y. Q.; Zheng, Q. Rheological behavior of biomimetic composite hydrogels with anisotropic structures. Acta Polymerica Sinica, 2024, 55(8), 1069-1080 DOI： 10.11777/j.issn1000-3304.2023.23275.

摘要

仿生构筑具有动态黏弹性行为的细胞外微环境，是组织工程领域细胞外基质(ECM)设计的重要策略. 本研究通过定向冷冻和限域拉伸干燥再溶胀的协同策略构建了具有各向异性结构的聚乙烯醇(PVA)/纤维素纳米纤维(CNF)复合水凝胶. 所制备的复合水凝胶呈现出平行于定向冷冻方向的取向纤维排列结构. 流变学实验结果表明，PVA/CNF复合水凝胶具有各向异性的动态模量(

G′

∥

= (76.77±1.61) kPa和

G′

⊥

= (42.93±1.34) kPa；

G''

∥

(5.44±0.26) kPa和

G''

⊥

= (3.71±0.13) kPa)，其储能模量(

G′

)和损耗模量(

G''

)在低频率和低应变区域随PVA含量的增加而增大，随CNF含量的增加呈现先增大后减小的变化趋势. 应力松弛实验结果表明，复合水凝胶基质具有黏弹性特性. 本文所总结的具有各向异性结构复合水凝胶的黏弹性性质及其规律，对于指导组织工程仿生ECM的设计及其对细胞生物行为的影响方面具有潜在的应用价值.

Abstract

Biomimetic construction of extracellular microenvironment with dynamic viscoelastic behavior is an important strategy for the design of extracellular matrix (ECM) in tissue engineering. In this study

a poly(vinyl alcohol) (PVA)/cellulose nanofiber (CNF) composite hydrogel with anisotropic structure was constructed through the cooperative strategy of directional freezing (DF) and confined drying and re-swelling (CDR). The prepared composite hydrogel showed the oriented fiber arrangement structure parallel to the directional freezing direction. Rheological experiments showed that PVA/CNF composite hydrogel had anisotropic dynamic modulus (

∥

= (76.77±1.61) kPa and

⊥

= (42.93±1.34) kPa;

∥

(5.44±0.26) kPa and

⊥

= (3.71±0.13) kPa). The energy storage modulus (

') and loss modulus (

'') improved with the increase of PVA content in the low frequency and low strain regions

and showed a tendency of increasing and then decreasing with the increase of CNF content. The results of stress relaxation experiments indicated that the composite hydrogel had viscoelastic properties. Therefore

the viscoelastic pro

perties and rules of the composite hydrogel with anisotropic structures summarized in this study have potential applications in guiding the design of tissue-engineered biomimetic ECM and its effect on cell biological behavior.

关键词

各向异性复合水凝胶细胞外基质流变行为应力松弛

Keywords

AnisotropyComposite hydrogelExtracellular matrixRheological behaviorStress relaxation

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