Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures

Xue-hui Zhang; Yan-qin Wang; Qiang Zheng

doi:10.11777/j.issn1000-3304.2023.23275

您当前的位置：

首页 >

文章列表页 >

Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures

更新时间：2024-05-07

- Rheological Behavior of Biomimetic Composite Hydrogels with Anisotropic Structures
- Acta Polymerica Sinica Pages: 1-12(2024)
- 作者机构：
  
  1.太原理工大学，材料科学与工程学院，浙江大学高分子科学与工程学系杭州 310027
  2.(太原理工大学，生物医学工程学院太原 030024) (，浙江大学高分子科学与工程学系杭州 310027)
  3.(太原理工大学，山西浙大新材料与化工研究院太原 030001) (，浙江大学高分子科学与工程学系杭州 310027)
  4.太原理工大学，高分子合成与功能构造教育部重点实验室，浙江大学高分子科学与工程学系杭州 310027
- 作者简介：
  
  Yan-qin Wang, E-mail: wangyanqin@tyut.edu.cn
  Qiang Zheng, E-mail: zhengqiang@zju.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2023.23275
  CLC：
- Published Online：07 May 2024，
  
  Received：30 November 2023，
  
  Accepted：22 March 2024
扫描看全文
张雪慧, 王艳芹, 郑强. 仿生各向异性结构复合水凝胶及其流变学行为. 高分子学报, doi: 10.11777/j.issn1000-3304.2023.23275

Zhang, X. H.; Wang, Y. Q.; Zheng, Q. Rheological behavior of biomimetic composite hydrogels with anisotropic structures. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2023.23275
张雪慧, 王艳芹, 郑强. 仿生各向异性结构复合水凝胶及其流变学行为. 高分子学报, doi: 10.11777/j.issn1000-3304.2023.23275 DOI：

Zhang, X. H.; Wang, Y. Q.; Zheng, Q. Rheological behavior of biomimetic composite hydrogels with anisotropic structures. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2023.23275 DOI：

摘要

仿生构筑具有动态黏弹性行为的细胞外微环境，是组织工程领域细胞外基质(ECM)设计的重要策略. 本研究通过定向冷冻和限域拉伸干燥再溶胀的协同策略构建了具有各向异性结构的聚乙烯醇(PVA)/纤维素纳米纤维(CNF)复合水凝胶. 所制备的复合水凝胶呈现出平行于定向冷冻方向的取向纤维排列结构. 流变学实验结果表明，PVA/CNF复合水凝胶具有各向异性的动态模量(

G′

∥

= (76.77±1.61) kPa和

G′

⊥

= (42.93±1.34) kPa；

G''

∥

(5.44±0.26) kPa和

G''

⊥

= (3.71±0.13) kPa)，其储能模量(

G′

)和损耗模量(

G''

)在低频率和低应变区域随PVA含量的增加而增大，随CNF含量的增加呈现先增大后减小的变化趋势. 应力松弛实验结果表明，复合水凝胶基质具有黏弹性特性. 本文所总结的具有各向异性结构复合水凝胶的黏弹性性质及其规律，对于指导组织工程仿生ECM的设计及其对细胞生物行为的影响方面具有潜在的应用价值.

Abstract

Biomimetic construction of extracellular microenvironment with dynamic viscoelastic behavior is an important strategy for the design of extracellular matrix (ECM) in tissue engineering. In this study

a poly(vinyl alcohol) (PVA)/cellulose nanofiber (CNF) composite hydrogel with anisotropic structure was constructed through the cooperative strategy of directional freezing (DF) and confined drying and re-swelling (CDR). The prepared composite hydrogel showed the oriented fiber arrangement structure parallel to the directional freezing direction. Rheological experiments showed that PVA/CNF composite hydrogel had anisotropic dynamic modulus (

∥

= (76.77±1.61) kPa and

⊥

= (42.93±1.34) kPa;

∥

(5.44±0.26) kPa and

⊥

= (3.71±0.13) kPa). The energy storage modulus (

') and loss modulus (

'') improved with the increase of PVA content in the low frequency and low strain regions

and showed a tendency of increasing and then decreasing with the increase of CNF content. The results of stress relaxation experiments indicated that the composite hydrogel had viscoelastic properties. Therefore

the viscoelastic properties and rules of the composite hydrogel with anisotropic structures summarized in this study have potential applications in guiding the design of tissue-engineered biomimetic ECM and its effect on cell biological behavior.

关键词

各向异性复合水凝胶细胞外基质流变行为应力松弛

Keywords

AnisotropyComposite hydrogelExtracellular matrixRheological behaviorStress relaxation

references

Nancy K.; Sina K.; Eugenia K. Structurally anisotropic hydrogels for tissue engineering. Trends Chem., 2021, 3(12), 1002-1026. doi:10.1016/j.trechm.2021.09.009http://dx.doi.org/10.1016/j.trechm.2021.09.009

Lou J. Z.; Mooney D. J. Chemical strategies to engineer hydrogels for cell culture. Nat. Rev. Chem., 2022, 6(10), 726-744. doi:10.1038/s41570-022-00420-7http://dx.doi.org/10.1038/s41570-022-00420-7

Li J. W.; Chen G. J.; Xu X. Q.; Abdou P.; Jiang Q.; Shi D. Q.; Gu Z. Advances of injectable hydrogel-based scaffolds for cartilage regeneration. Regen. Biomater., 2019, 6(3), 129-140. doi:10.1093/rb/rbz022http://dx.doi.org/10.1093/rb/rbz022

Ma Y. F.; Han T.; Yang Q. X.; Wang J.; Feng B. C.; Jia Y. B.; Wei Z.; Xu F. Viscoelastic cell microenvironment: Hydrogel-based strategy for recapitulating dynamic ECM mechanics. Adv. Funct. Mater., 2021, 31(24), 2100848. doi:10.1002/adfm.202100848http://dx.doi.org/10.1002/adfm.202100848

Wang Y. Q.; Zhang X. H.; Wang J. H.; Fan Y. B. Viscoelastic modeling of the stress relaxation behavior for the bionic extracellular matrix polymer scaffold. Med. Nov. Technol. Devices, 2022, 16, 100181. doi:10.1016/j.medntd.2022.100181http://dx.doi.org/10.1016/j.medntd.2022.100181

Hang J. T.; Xu G. K.; Gao H. J. Frequency-dependent transition in power-law rheological behavior of living cells. Sci. Adv., 2022, 8(18), eabn6093. doi:10.1126/sciadv.abn6093http://dx.doi.org/10.1126/sciadv.abn6093

Zuidema J. M.; Rivet C. J.; Gilbert R. J.; Morrison F. A. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J. Biomed. Mater. Res. Part B Appl. Biomater., 2014, 102(5), 1063-1073. doi:10.1002/jbm.b.33088http://dx.doi.org/10.1002/jbm.b.33088

Chaudhuri O.; Cooper-White J.; Janmey P. A.; Mooney D. J.; Shenoy V. B. Effects of extracellular matrix viscoelasticity on cellular behaviour. Nature, 2020, 584(7822), 535-546. doi:10.1038/s41586-020-2612-2http://dx.doi.org/10.1038/s41586-020-2612-2

Park J. S.; Burckhardt C. J.; Lazcano R.; Solis L. M.; Isogai T.; Li L. Q.; Chen C. S.; Gao B. N.; Minna J. D.; Bachoo R.; DeBerardinis R. J.; Danuser G. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature, 2020, 578(7796), 621-626. doi:10.1038/s41586-020-1998-1http://dx.doi.org/10.1038/s41586-020-1998-1

Zhou Z. P.; Deng T.; Tao M. X.; Lin L. S.; Sun L. Y.; Song X. M.; Gao D. X.; Li J. X.; Wang Z. J.; Wang X. Z.; Li J. P.; Jiang Z. X.; Luo L.; Yang L.; Wu M. Y. Snail-inspired FAG/GelMA hydrogel accelerates diabetic wound healing via inflammatory cytokines suppression and macrophage polarization. Biomaterials, 2023, 299(8), 122141. doi:10.1016/j.biomaterials.2023.122141http://dx.doi.org/10.1016/j.biomaterials.2023.122141

Fu M.; Sun Z. X.; Liu X. B.; Huang Z. K.; Luan G. F.; Chen Y. T.; Peng J. P.; Yue K. Highly stretchable, resilient, adhesive, and self-healing ionic hydrogels for thermoelectric application. Adv. Funct. Mater., 2023, 33(43), 2306086. doi:10.1002/adfm.202306086http://dx.doi.org/10.1002/adfm.202306086

Arno M. C.; Inam M.; Weems A. C.; Li Z. H.; Binch A. L. A.; Platt C. I.; Richardson S. M.; Hoyland J. A.; Dove A. P.; O'Reilly R. K. Exploiting the role of nanoparticle shape in enhancing hydrogel adhesive and mechanical properties. Nat. Commun., 2020, 11(1), 1420. doi:10.1038/s41467-020-15206-yhttp://dx.doi.org/10.1038/s41467-020-15206-y

刘水莲, 周洋, 陈福花, 朱寿进, 宿烽, 李速明. 新型羧甲基壳聚糖水凝胶流变性能, 药物释放及细胞相容性研究. 化学学报, 2015, 73(1), 47-52.

Goncharuk V. V.; Dubrovina L. V. Rheological properties and water-retaining power of agar hydrogels with carboxymethyl cellulose. Russ. J. Appl. Chem., 2020, 93(7), 1019-1026. doi:10.1134/s1070427220070113http://dx.doi.org/10.1134/s1070427220070113

Steel E. M.; Azar J. Y.; Sundararaghavan H. G. Electrospun hyaluronic acid-carbon nanotube nanofibers for neural engineering. Materialia, 2020, 9, 100581. doi:10.1016/j.mtla.2019.100581http://dx.doi.org/10.1016/j.mtla.2019.100581

Zhang X. H.; Lang B.; Yu W. W.; Jia L.; Zhu F. B.; Xue Y. R.; Wu X. G.; Qin Y. X.; Chen W. Y.; Wang Y. Q.; Zheng Q. Magnetically induced anisotropic conductive hydrogels for multidimensional strain sensing and magnetothermal physiotherapy. Chem. Eng. J., 2023, 474, 145832. doi:10.1016/j.cej.2023.145832http://dx.doi.org/10.1016/j.cej.2023.145832

Dong X. Y.; Guo X.; Liu Q. Y.; Zhao Y. J.; Qi H. B.; Zhai W. Strong and tough conductive organo-hydrogels via freeze-casting assisted solution substitution. Adv. Funct. Mater., 2022, 32(31), 2203610. doi:10.1002/adfm.202203610http://dx.doi.org/10.1002/adfm.202203610

Zhang X. H.; Wang Y. Q.; Wu X. G.; Zhu F. B.; Qin Y. X.; Chen W. Y.; Zheng Q. A universal post-treatment strategy for biomimetic composite hydrogel with anisotropic topological structure and wide range of adjustable mechanical properties. Biomater. Adv., 2022, 133, 112654. doi:10.1016/j.msec.2022.112654http://dx.doi.org/10.1016/j.msec.2022.112654

Zhu Q. L.; Dai C. F.; Wagner D.; Daab M.; Hong W.; Breu J.; Zheng Q.; Wu Z. L. Distributed electric field induces orientations of nanosheets to prepare hydrogels with elaborate ordered structures and programmed deformations. Adv. Mater., 2020, 32(47), 2005567. doi:10.1002/adma.202005567http://dx.doi.org/10.1002/adma.202005567

Lin X. H.; Xing X.; Li S. S.; Wu X. Y.; Jia Q. Q.; Tu H.; Bian H. L.; Lu A.; Zhang L. N.; Yang H. Y.; Duan B. Anisotropic hybrid hydrogels constructed via the noncovalent assembly for biomimetic tissue scaffold. Adv. Funct. Mater., 2022, 32(21), 2112685. doi:10.1002/adfm.202112685http://dx.doi.org/10.1002/adfm.202112685

Zheng C. X.; Lu K. Y.; Lu Y.; Zhu S. L.; Yue Y. Y.; Xu X. W.; Mei C. T.; Xiao H. N.; Wu Q. L.; Han J. Q. A stretchable, self-healing conductive hydrogels based on nanocellulose supported graphene towards wearable monitoring of human motion. Carbohydr. Polym., 2020, 250, 116905. doi:10.1016/j.carbpol.2020.116905http://dx.doi.org/10.1016/j.carbpol.2020.116905

Pan Z. Z.; Nishihara H.; Iwamura S.; Sekiguchi T.; Sato A.; Isogai A.; Kang F. Y.; Kyotani T.; Yang Q. H. Cellulose nanofiber as a distinct structure-directing agent for xylem-like microhoneycomb monoliths by unidirectional freeze-drying. ACS Nano, 2016, 10(12), 10689-10697. doi:10.1021/acsnano.6b05808http://dx.doi.org/10.1021/acsnano.6b05808

Choi Y.; Cho D. H.; Kim S.; Kim H. J.; Park T. J.; Kim K. B.; Park Y. M. Synergistic enhancement of hydrogel adhesion via tough chemical bonding and physical entanglements. Polym. Test., 2022, 107, 107482. doi:10.1016/j.polymertesting.2022.107482http://dx.doi.org/10.1016/j.polymertesting.2022.107482

Lopes da Silva José, A. Thermorheological complex behaviour of maltosyl-chitosan derivatives in aqueous solution. React. Funct. Polym., 2012, 72(9), 657-666. doi:10.1016/j.reactfunctpolym.2012.06.011http://dx.doi.org/10.1016/j.reactfunctpolym.2012.06.011

梁子毅, 黄鸿键, 倪鹏, 徐仁凤, 王正朝, 刘海清. 贻贝黏附蛋白启发的甲壳素纳米晶须增强湿态黏附水凝胶的研究. 高分子学报, 2023, 54(3), 365-380.

Yan M. Z.; Cai J. Q.; Fang Z. Q.; Wang H.; Qiu X. Q.; Liu W. F. Anisotropic muscle-like conductive composite hydrogel reinforced by lignin and cellulose nanofibrils. ACS Sustain. Chem. Eng., 2022, 10(39), 12993-13003. doi:10.1021/acssuschemeng.2c02506http://dx.doi.org/10.1021/acssuschemeng.2c02506

Shi Y.; Xiong D.; Li J.; Wang N. In situ reduction of graphene oxide nanosheets in poly(vinyl alcohol) hydrogel by γ-ray irradiation and its influence on mechanical and tribological properties. J. Phys. Chem. C, 2016, 120(34), 19442-19453. doi:10.1021/acs.jpcc.6b05948http://dx.doi.org/10.1021/acs.jpcc.6b05948

Liu K. Z.; Han L.; Tang P. F.; Yang K. M.; Gan D. L.; Wang X.; Wang K. F.; Ren F. Z.; Fang L. M.; Xu Y. G.; Lu Z. F.; Lu X. An anisotropic hydrogel based on mussel-inspired conductive ferrofluid composed of electromagnetic nanohybrids. Nano Lett., 2019, 19(12), 8343-8356. doi:10.1021/acs.nanolett.9b00363http://dx.doi.org/10.1021/acs.nanolett.9b00363

Ma Y. Z.; Ma A. J.; Luo T.; Xiao S. Y.; Zhou H. W. Fabrication of anisotropic nanocomposite hydrogels by magnetic field-induced orientation for mimicking cardiac tissue. J. Appl. Polym. Sci., 2023, 140(1), e53248. doi:10.1002/app.53248http://dx.doi.org/10.1002/app.53248

秦晶晶, 刘丹, 高翔, 宁晓辉, 鲍琳, 李延. 瓜尔豆胶清洁胶的流变学性能研究. 湘潭大学学报(自然科学版), 2023, 45(3), 66-74.

谭魏葳, 雷苏苏, 龙涛, 徐志朗, 李德富, 穆畅道, 葛黎明. 具有葡萄糖响应性释药的多糖基可注射自愈合水凝胶. 高分子学报, 2023, 54(8), 1155-1165. doi:10.11777/j.issn1000-3304.2023.23024http://dx.doi.org/10.11777/j.issn1000-3304.2023.23024

Ai J. Y.; Li K.; Li J. B.; Yu F.; Ma J. Super flexible, fatigue resistant, self-healing PVA/xylan/borax hydrogel with dual-crosslinked network. Int. J. Biol. Macromol., 2021, 172, 66-73. doi:10.1016/j.ijbiomac.2021.01.038http://dx.doi.org/10.1016/j.ijbiomac.2021.01.038

Du C.; Zhang X. N.; Sun T. L.; Du M.; Zheng Q.; Wu Z. L. Hydrogen-bond association-mediated dynamics and viscoelastic properties of tough supramolecular hydrogels. Macromolecules, 2021, 54(9), 4313-4325. doi:10.1021/acs.macromol.1c00152http://dx.doi.org/10.1021/acs.macromol.1c00152

Mano J. F. Viscoelastic properties of bone: mechanical spectroscopy studies on a chicken model. Mater. Sci. Eng. C, 2005, 25(2), 145-152. doi:10.1016/j.msec.2005.01.017http://dx.doi.org/10.1016/j.msec.2005.01.017

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Glassy Hydrogels: From New State to High Performances of Gel Materials

Rheology Study on Polypropylene Blends with in situ Core-Shell Dispersed Particles

Research Progress in Anisotropic Thermal Conduction Behavior of Polyimide Films

Effect of Sodium Dodecyl Sulfate on the Rheological Behavior of Poly(vinyl alcohol) Aqueous Solution

Rheological Behavior of Hydroxyethylacrylate/Sodium Acryloyldimethyl Taurate Copolymer Aqueous Solution

Related Author

Zi-liang Wu

Qiang Zheng

Xin-ning Zhang

Cong Du

Jia-yu Hu

Feng Chen

Ming-zhao Li

Liang-hai Zhu

Related Institution

College of Materials Science and Engineering, Qingdao University

Department of Polymer Science and Engineering, Zhejiang University

College of Chemistry and Materials Engineering, Zhejiang Agriculture & Forest University

International Research Center for X Polymers, Zhejiang University, Haining

MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University

Postal code：100190
Tel：010-62588927 Email：gfzxb@iccas.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备05002797号-8 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰