Active acrylic esters of 8-hydroxyquinoline

8-acryloxy-quinoline (AQ) and 8-me-thacryloxy-quinoline (MAQ) were synthesized from 8-hydroxyquinoline and corresponding acryl chlorides and polymerized. These two polymers

due to the neighbouring group effect of nitrogen atom on the quinolyl ring

are reactive towards ammonolysis or alcoholysis reactions under mild condition and therefore

can be used to couple proteins. Stirring p(AQ) or p(MAQ) with glucoamylase in borate buffer of pH 7 at room temperature

immolbilized enzyme with specific activity of 5328μ/g and 4320μ/g were obtained respectively.