A new method for preparing crosslinked fibrous gelatin membrane was developed in order to circumvent the disadvantages of traditional methods for crosslinking electrospun gelatin fibers.Through a series of experiments

the stable window of one-step electrospinning was found as 3 mL of 10% gelatin solution in TFE with 40 μL glutaraldehyde added.SEM micrographs showed the diameter of the electrospun fibers ranged from 1 μm to 1.5 μm and was thicker than that of the noncrosslinked gelatin fibers.Furthermore

a phenomenon of false core-shell fibers was observed

which might be attributed to the rapid evaporation of glutaraldehyde at the vicinity of the fiber surface.Rheological behavior of the electrospinning solution was studied

indicating that viscosity had a drastic rise upon adding glutaraldehyde and maintained slow increase after 10 min.It was also found that a fibrous structure of the electrospun gelatin membrane could still be preserved after soaked in distilled water at 37℃ although the membrane suffered from about 10% weight loss.The one-step electrospinning method should be useful in the fields of biomedical engineering

such as tissue engineering and wound healing.