A series of galactose glycosylated thermoresponsive hydrogel (P(NIPAAm-co-GAC)) and chitosan glycosylated thermoresponsive hydrogel (P(NIPAAm-co-CSA)) were synthesized by free radical polymerization with different ratios. The temperature responsibility of the copolymerized hydrogels was investigated. The lower critical solution temperature (LCST) of P(NIPAAm-co-GAC) hydrogels was 30～35℃ in distilled water and cell culture media. However

the LCST of P(NIPAAm-co-CSA) hydrogels was 30～35℃ in distilled water and lower than 30℃ in cell culture media

respectively. Furthermore

behaviors of HepG2 cell cultured on the P(NIPAAm-co-GAC)

P(NIPAAm-co-CSA)and poly(N-isopropylacrylamide) (PNIPAAm) hydrogels were investigated. Cell mo~rph~olo~gies on different hydrogels scaffolds were photographed under a phase contrast microscope equipped with a digital camera. Investigation of the growth behaviors and viability of HepG2 cells on different hydrogels indicated that HepG2 cell grew very well on the surface of P(NIPAAm-co-GAC) and PNIPAAm hydrogels. However

the growth of HepG2 cell was inhibited on the surface of P(NIPAAm-co-CSA) hydrogels. The 3-(4

5-dimethy thioazol-2-yl)-2

5-di-phenytetrazoliumromide (MTT) assay was used to quantify the cell viability of HepG2 on different hydrogels. Cell viability on P(NIPAAm-co-GAC) hydrogels was 10 times higher than that on P(NIPAAm-co-CSA) hydrogels after 6 day’s incubation. Moreover

the viability of HepG2 cell increased with increasing the amount of introduced galactose. On the contrary

the viability of HepG2 cell decreased with increasing the amount of introduced chitosan. Investigation of the temperature induced detachment indicated that the cell could be spontaneously detached from the surface of poly(NIPAAm-co-GAC) hydrogels by lowing temperature

which could avoid the damage to the cells caused by enzymatic digestion. The detachment rate of cell sheets was much faster than that of single cells

which was indicated by the detachment rate of 64% for cell sheets and 32% for single cells after 10 min at low temperature. Investigation of cells transshipment indicated that the viability of cells obtained by temperature induced detachment was much higher than that obtained by enzymatic digestion. In conclusion

the introduction of chitosan into PNIPAAm was not favorable for the growth of HepG2

but the incorporation of galactose greatly facilitated its growth. Further more

intact cell sheets could be obtained by controlling the environmental temperature. All these results provided a novel method and experimental basis for developing materials used in tissue engineering.