4-Vinyl pyridine (4VP) was grafted on micron-sized silica gel particles in a manner of graft from in a solution poltmerization system by right of the mediation of the coupling agent 3-methacryloxypropyltrimethoxysilane (MPS)

obtaining the grafted functional particles P4VP/SiO2.Subsequently

the molecular imprinting towards the grafted P4VP was conducted with L-Asp that is one enantiomer of aspartic acid (Asp) as template molecule and 1

6-dibromohexane as crosslinking agent by using the novel surface-molecular imprinting technique put forward and established by our research group

and the L-Asp molecule-imprinted material MIP-P4VP/SiO2 was prepared.With another enantiomer of aspartic acid

D-Asp

as a contrast compound

both static method and dynamic method were adopted to study the recognition character of MIP-P4VP/SiO2 for L-Asp

and the separation ability of MIP-P4VP/SiO2 towards L-Asp and D-Asp in the raceme was explored.The experiment results show that MIP-P4VP/SiO2 have obvious recognition selectivity and fine binding affinity for L-Asp

and the selectivity coefficient is equal to 3.85 with respect to D-Asp

displaying the fine separation property for the enantiomers of aspartic acid.Besides

MIP-P4VP/SiO2 has excellent elution property

with a diluted aqueous solution of NaOH as the eluent

the desorption ratios of L-Asp reach 98.52% in 12 BV.