The fluorescence enhancement of green fluorescence protein (GFP) chromophore was realized through the self-assembly of amphiphilic block polymer and GFP chromophore.GFP chromophore and a series of amphiphilic poly(ethylene glycol)-b-poly(methyl methacrylate) (PEG-b-PMMA) with different PMMA lengths were synthesized.1H and 13C nuclear magnetic resonance (1H and 13C-NMR)
high-resolution mass spectroscopy (HRMS)
Fourier transform infrared spectroscopy (FTIR)
gel permeation chromatography (GPC)
ultraviolet-visible absorption (UV-Vis) and fluorescent spectroscopy were applied to study the structure and properties of chromophore and polymers.The maximum UV-Vis absorption peak and fluorescent emission peak were at about 371 nm and 428 nm
respectively.After the self-assembly of PEG-b-PMMA with chromophore
the UV-Vis absorption of chromophore disappeared while the fluorescent emission intensity increased markedly with a red-shift of 70 nm
which could be attributed to the chromophore's rotation restriction in polymeric nanoparticles.The size and shape of nanoparticles were measured by dynamic light scattering (DLS) and transmission electron microscopy (TEM).Benefiting from the appropriate size and good fluorescent property
these PEG-b-PMMA/chromophore nanoparticles were used in cell imaging successfully.
关键词
绿色荧光蛋白荧光生色团两亲性嵌段聚合物自组装成像
Keywords
Green fluorescent proteinChromophoreAmphiphilic polymerSelf-assemblyImaging
SYNTHESIS AND OPTICAL PROPERTIES OF GFP-MIMIC FLUORESCENT POLYMER
Structured Liquids: Design, Construction and Applications
Synthesis and Self-assembly of Asymmetric Molecular Brushes with Azobenzene-containing Side Chains
Preparation and Properties of Dopamine Modified Polydiacetylene Composite Thermochromic Material
Synthesis and Self-assembly Behavior of Porphyrin-based Polypeptides
Related Author
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Related Institution
School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240
Instrumental Analysis Center, Shanghai Jiao Tong University
State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology
Department of Polymer Science and Engineering, University of Massachusetts, Amherst
Shanghai Key Laboratory of Advanced Polymeric Materials, School of Materials Science and Engineering, East China University of Science and Technology