The copolymer PMT82 was synthesized by free radical polymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) and trimethoxysilylpropyl methacrylate (TSMA) with the monomer ratio of 8∶2.The copolymer PMT82 was dip-coated onto glutaraldehyde-crosslinked chitosan (CS-GA) surfaces to prepare phosphorylcholine-coated CS-GA film (CS-GA-PMT82a) and then stabilized by the amine-catalyzed crosslinking reactions of the trimethoxysilane groups

obtaining the phosphorylcholine-crosslinked CS-GA surfaces (CS-GA-PMT82b).The hydrophilicity and elementary composition of the modified surfaces of were characterized by dynamic contact angle (DCA) measurements and X-ray photoelectron spectroscopy (XPS).The obvious decrease in the contact angles and the new signals for P2p

Si2p and N+(CH3)3 provided strong evidences for the successful establishment of PMT82 on the CS-GA surface.The anticoagulation property of the surfaces was estimated by platelet adhesion measurements.Compared with the chitosan films

the CS-GA-PMT82b surfaces massively reduced the platelet adhesion

indicating that the cell outer membrane mimetic structures were formed on the modified surfaces with PMT82 as the coat

which led to the significantly improved anticoagulation property of the modified surfaces of the composite material.This facile method of fabricating the stable cell outer membrane mimetic surfaces by dip-coating and catalytic crosslinking may have potential applications in the fields of anticoagulation property coatings

drug delivery

and tissue engineering.