The Langmuir-Blodgett (LB) technique was used to prepare thin films with cell outer membrane mimetic structure on the surface of cover glass.A crosslinkable amphiphilic random copolymer PMST

which was synthesized with acrylate monomers 2-methacryloyloxyethyl phosphorylcholine (MPC)

stearyl methacrylate (SMA) and 3-trimethoxysilylpropyl methacrylate (TSMA) by free radical polymerization in solution

was applied to prepare the mononlayer

bilayer

and trilayer LB films according to the Y-type deposition mode.The surface hydrophilicity and surface morphology were measured by dynamic contact angles (DCA) and atomic force microscope (AFM) methods.The PMST polymer formed a dense and regular film on the surface of the cover glass

and the density of film increased with the number of layers.The surface hydrophilicity of monolayer and trilayer film was similar

and the bilayer was better than them in hydrophilicity.The difference can be attributed to the different surface structure:the bilayer film has the cell outer membrane mimetic structure and the other two have a reversed structure of the cell outer membrane.Once it contacts with water

the film surface structure will change as a result of the migration and reorientation of surface groups at the gas/water interface.