壳聚糖在离子液体中均相接枝聚乙烯亚胺及其基因转运

陈会英; 张树彪; 彭孝军; 崔韶晖; 赵轶男; 张传敏; 韩颖

doi:10.11777/j.issn1000-3304.2014.13461

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壳聚糖在离子液体中均相接枝聚乙烯亚胺及其基因转运

研究论文 | 更新时间：2021-03-19

- 壳聚糖在离子液体中均相接枝聚乙烯亚胺及其基因转运
- Homogeneous Graft of Chitosan with Polyethylenimine in Ion Liquid and Its Application in Gene Delivery
- 高分子学报 2014年第8期页码：1089-1095
- 作者机构：
  
  1. 大连理工大学化工与环境生命学部精细化工国家重点实验室,大连,116024
  2. 大连民族学院生命科学学院国家民委-教育部生物资源与技术利用国家重点实验室,大连,116600
- 作者简介：
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2014.13461
  中图分类号：
- 纸质出版日期：2014-8-20，
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陈会英, 张树彪, 彭孝军, 崔韶晖, 赵轶男, 张传敏, 韩颖. 壳聚糖在离子液体中均相接枝聚乙烯亚胺及其基因转运[J]. 高分子学报, 2014,(8):1089-1095.

Hui-ying Chen, Shu-biao Zhang, Xiao-jun Peng, Shao-hui Cui, Yi-nan Zhao, Chuan-min Zhang, Ying Han. Homogeneous Graft of Chitosan with Polyethylenimine in Ion Liquid and Its Application in Gene Delivery[J]. Acta Polymerica Sinica, 2014,(8):1089-1095.
陈会英, 张树彪, 彭孝军, 崔韶晖, 赵轶男, 张传敏, 韩颖. 壳聚糖在离子液体中均相接枝聚乙烯亚胺及其基因转运[J]. 高分子学报, 2014,(8):1089-1095. DOI： 10.11777/j.issn1000-3304.2014.13461.

Hui-ying Chen, Shu-biao Zhang, Xiao-jun Peng, Shao-hui Cui, Yi-nan Zhao, Chuan-min Zhang, Ying Han. Homogeneous Graft of Chitosan with Polyethylenimine in Ion Liquid and Its Application in Gene Delivery[J]. Acta Polymerica Sinica, 2014,(8):1089-1095. DOI： 10.11777/j.issn1000-3304.2014.13461.

摘要

以离子液体1-丁基-3-甲基咪唑氯盐（[BMIM]Cl）为反应介质和催化剂，采用羰基二咪唑为偶联试剂，均相合成了壳聚糖/聚乙烯亚胺接枝共聚物（CS-g-PEI）.采用红外光谱、核磁共振氢谱等分析手段对接枝共聚物进行了表征，证实了聚乙烯亚胺在壳聚糖分子链上发生了接枝共聚，接枝率达到42%.采用复凝聚的方法制备了CS-g-PEI/DNA纳米复合物，并利用动态光散射、透射电镜、凝胶电泳等技术对CS-g-PEI/DNA复合物的物理化学性质进行了表征.以人宫颈癌Hep-2细胞为宿主细胞、pGL3荧光蛋白为报告基因，考察了CS-g-PEI/DNA复合物的体外转染性能.研究发现CS-g-PEI介导的荧光素酶质粒的表达较壳聚糖介导的荧光素酶的表达提高了大约160倍.MTT毒性实验表明，CS-g-PEI/DNA复合物在实验浓度范围内细胞毒性比较低.

Abstract

By using ionic liquid 1-butyl-3-methyl imidazolium chloride ([BMIM]Cl) as reaction solvent and catalyst

and 1

1-carbonyldiimidazole as a coupling reagent

chitosan grafted with low molecular weight polyethylenimine (CS-g-PEI) was synthesized in a non-aqueous homogeneous solution.1H-NMR and FTIR experiments characterized the composition of copolymer CS-g-PEI and confirmed that the chitosan chains were successfully grated with polyethylenimine at a ratio of 42%.By using a coagulation method

CS-g-PEI/DNA complex nanoparticles were obtained

and their physicochemical properties were characterized with transmission electron microscopy (TEM)

dynamic light scattering (DLS) and Agarose gel electrophoresis assay.The transfection efficiency of CS-g-PEI was evaluated in Hep-2 cell lines with pGL3 as reporting genes.The gene expressing mediated by CS-g-PEI was 160 times higher than that by CS.MTT assays results showed that CS-g-PEI had low cytotoxicity in the range of the experimental dose.The results indicate that CS-g-PEI synthesized in ion liquid [BMIM]Cl has a very good potential as efficient and safe non-viral gene vectors.

关键词

非病毒基因载体壳聚糖离子液体聚乙烯亚胺接枝聚合物

Keywords

Non-viral gene vectorChitosanIonic liquidPEIGraftcopolymer

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