A novel peptide-grafted polymer P-GS5 was synthesized by modification of poly(D

L-lactide-co-β-malic acid) (P-COOH) with pentapeptides Gly-Arg-Gly-Asp-Ser (GRGDS)

and its biocompatibility was investigated.Infrared spectroscopy (IR)

element analysis (EA) and X-ray photoelectron spectroscopy (XPS) were used to characterize the synthesized materials (poly(D

L-lactide) (PDLLA)

P-COOH

P-GS5).Hydrophilic properties of these polymers were investigated by contact angle measurements (CA).Subsequently

biocompatibility experiments were conducted by testing human umbilical cord vein endothelial cell (HUVEC) (24 h) and platelet (30 min) adhesion on polymer surfaces.The number of endothelial cell and platelet adhesion was counted by cytometer and automatic hematological analyzer

respectively.It has been shown that the hydrophilicity of P-GS5 was greatly improved.Water contact angle was 84° for PDLLA

78° for P-COOH and 58° for P-GS5.Cell adhesion data indicated that P-GS5 could promote endothelial cell attachments.The adhesion ratio of PDLLA

P-COOH and P-GS5 was 45%

62% and 109%

respectively.According to the platelet adhesion results

GRGDS on P-GS5 did not induce platelet aggregation.The platelet adhesion capacity of P-GS5 was much lower than that of the other two polymers

while the adhesion ratio of PDLLA

P-COOH and P-GS5 was 47%

37% and 18%

respectively.In conclusion

P-GS5 displayed improved hydrophilicity

higher affinity to endothelial cell and better ability of anti-platelet adhesion compared with PDLLA and P-COOH.It could be a potential biomedical polymer.