A novel series of anionic fluorine-containing amphiphilic graft copolymers P(HFMA-co-NaSS)-g-PEG were synthesized by graft copolymerization of poly(ethylene glycol) methyl ether methacrylate (MPEGMA) macromonomer

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4-hexafluorobutyl methacrylate (HFMA) and sodium 4-vinylbenzenesulfonate (NaSS).The molecular structures of the amphiphilic graft copolymer were characterized by FTIR

1H-NMR and 19F-NMR.The critical micelle concentration (CMC) of P(HFMA-co-NaSS)-g-PEG was measured by surface tension technique

indicating that the CMC increased gradually with increasing PEG branched chains and anionic contents in the copolymer.The average hydrodynamic diameter and size distribution of micelles were measured by dynamic light scattering (DLS).The measurement showed that the hydrodynamic diameter increased gradually with increasing the concentration of P(HFMA-co-NaSS)-g-PEG aqueous solution and the content of PEG branched chains in P(HFMA-co-NaSS)-g-PEG.The fluorescence spectrum showed that there was a significant interaction between P(HFMA-co-NaSS)-g-PEG micelles and bovine serum albumin (BSA).The fluorescence intensity of BSA decreased gradually in P(HFMA-co-NaSS)-g-PEG/BSA aqueous solution with increasing the concentration of P(HFMA-NaSS)-g-PEG aqueous solution

HFMA segments and NaSS contents.Transmission electron microscopy (TEM) was used to investigate P(HFMA-co-NaSS)-g-PEG micelles and the aggregates of P(HFMA-co-NaSS)-g-PEG/BSA

the results showed that P(HFMA-co-NaSS)-g-PEG copolymers could self-assemble into core-shell structure micelles.Besides

with the decrease of HFMA contents in the copolymer

the morphology of P(HFMA-co-NaSS)-g-PEG micelles changed from a leaf-like structure to a spherical one.In particular

when BSA was added

the micelles changed from a tight core-shell structure into a loose vesicle-like structure

and the micelle size and size distribution also increased.TEM micrographs of P(HFMA-co-NaSS)-g-PEG/BSA displayed a visible adsorption phenomenon between P(HFMA-co-NaSS)-g-PEG micelles and BSA

and suggested that P(HFMA-co-NaSS)-g-PEG may have potential applications in loading proteins and other bioactive drugs.