Poly(lactide-co-glycolide) (PLGA) porous scaffolds were prepared via room-temperature molding/particle leaching method

and in vitro degradations of those porous scaffolds were examined in phosphate buffer saline (PBS) solution at 37 ℃ for 24 weeks.Each PLGA85/15 scaffold of porosity a bit above 90% and weight about 50 mg was immersed in 10 mL PBS.The degradation media were replaced by fresh ones weekly.Part of degradation media were collected at series of degradation time points

and then added into the culture medium of mesenchymal stem cells (MSCs).Cell viability and osteogenic differentiation were detected via lactate dehydrogenase assay

Live/Dead staining

3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide assay

alkaline phosphatase staining and quantification.The results indicated that molecular weight of remaining PLGA

and weight

dimension

pore size

and interpore connectivity of scaffolds changed with different degrees.The degradation media at all of degradation times up to 24 weeks exhibited no severe cytotoxicity

and no significant negative influences on viability

proliferation and osteogenic differentiation of MSC were observed under the present conditions and examined period.