Rational Design of Tadpole-like Single-chain Particle Brush with Anti-protein and Antibacterial Performance

Jie Zhu; Nai-bing Wu; Xiao-ya Zhao; Yan-bin Fan; Xia-yun Huang; Dao-yong Chen

doi:10.11777/j.issn1000-3304.2023.23303

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Rational Design of Tadpole-like Single-chain Particle Brush with Anti-protein and Antibacterial Performance

更新时间：2024-04-18

- Rational Design of Tadpole-like Single-chain Particle Brush with Anti-protein and Antibacterial Performance
- Acta Polymerica Sinica Pages: 1-10(2024)
- 作者机构：
  
  1.聚合物分子工程国家重点实验室复旦大学高分子科学系上海 200438
  2.陶氏化学公司上海 201203
- 作者简介：
  
  Xia-yun Huang, E-mail: huangxiayun@fudan.edu.cn
  Dao-yong Chen, E-mail: chendy@fudan.edu.cn
- 基金信息：
- DOI：10.11777/j.issn1000-3304.2023.23303
  CLC：
- Published Online：18 April 2024，
  
  Received：30 December 2023，
  
  Accepted：15 March 2024
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朱杰, 吴乃冰, 赵晓雅, 范艳斌, 黄霞芸, 陈道勇. 具有抗蛋白黏附与抗菌性能的蝌蚪状单链粒子刷的构筑. 高分子学报, doi: 10.11777/j.issn1000-3304.2023.23303

Zhu, J.; Wu, N. B.; Zhao, X. Y.; Fan, Y. B.; Huang, X. Y.; Chen, D. Y. Rational design of tadpole-like single-chain particle brush with anti-protein and antibacterial performance. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2023.23303
朱杰, 吴乃冰, 赵晓雅, 范艳斌, 黄霞芸, 陈道勇. 具有抗蛋白黏附与抗菌性能的蝌蚪状单链粒子刷的构筑. 高分子学报, doi: 10.11777/j.issn1000-3304.2023.23303 DOI：

Zhu, J.; Wu, N. B.; Zhao, X. Y.; Fan, Y. B.; Huang, X. Y.; Chen, D. Y. Rational design of tadpole-like single-chain particle brush with anti-protein and antibacterial performance. Acta Polymerica Sinica, doi: 10.11777/j.issn1000-3304.2023.23303 DOI：

摘要

基于蝌蚪状单链粒子与硅片表面的多位点协同非共价作用，发展了一种兼具操作便捷性和优异“生物防污”性能的PEG刷接枝策略. 通过原子转移自由基聚合(ATRP)制备了聚乙二醇-

-聚(4-乙烯基吡啶) (PEG-

-P4VP)嵌段共聚物，利用“静电介导”法对PEG-

-P4VP的P4VP嵌段进行链内交联，进一步加入过量的碘乙烷，制备得到P4VP“头部”完全季铵化的蝌蚪状单链粒子(TSCP-Q). 在简单的溶液浸泡接枝过程中，充分交联塌缩的“头部”与硅片表面通过多位点非共价协同作用，显著减少其在表面的吸附面积，而充分舒展的PEG“尾部”则通过体积排斥效应，确保了TSCP-Q刷在表面的接枝均匀性. 因此，所获得的TSCP-Q刷可在硅片表面实现了稳定且均匀地接枝，其中PEG“尾部”位于P4VP“头部”锚定层上方并有效遮蔽锚定层电荷. 实验表明，TSCP-Q刷具有优异的抗牛血清蛋白(BSA)黏附性能(4.8 ng/cm

)，并且能够有效抑制金黄色葡萄球菌(

S. aureus

)的繁殖(抗菌率可达81%).

Abstract

Based on the synergistic non-covalent interaction of tadpole-like single-chain particles with multiple sites on the wafer surface

a PEG brush grafting strategy has been developed that combines ease of handling and excellent "biofouling" properties. Specifically

the block copolymer poly(ethylene glycol)-

-poly(4-vinylpyridine) (PEG-

-P4VP) was synthesized by atom transfer radical polymerization (ATRP). The P4VP block was then intra-chain cross-linked using the "electrostatic-mediated" strategy

and the P4VP "head" was completely quaternized by further addition of excess ethyl iodide

yielding tadpole-like single-chain particles with completely quaternized "heads" (TSCP-Q). By simple solution immersion grafting method

the completely cross-linked and collapsed "head" significantly reduces its adsorption area on the silicon wafer through multi-site non-covalent synergistic interaction

while the extended PEG "tail" ensures grafting uniformity of TSCP-Q brushes on the surface through volume repulsion effect. As a result

the obtained TSCP-Q brushes achieve stable and uniform grafting on the silicon wafer

with PEG "tail" overlying the P4VP "head" anchoring layer and effectively masking the charge of the anchoring layer. The TSCP-Q brushes were shown to have excellent anti-bovine serum albumin (BSA) adsorption (4.8 ng/cm

) and could effectively inhibit the colonization of

Staphylococcus aureus

(

S. aureus

) (killing efficacy up to 81%).

关键词

蝌蚪状单链粒子聚合物刷聚乙二醇抗蛋白黏附抗菌

Keywords

Tadpole-like single-chain particlePolymer brushPoly(ethylene glycol)Anti-protein adsorptionAntibacterial

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