Preparation of a biperous polymeric stationary phase for high-speed flowthrough chromatography was reported Using calcium carbonate suspension(200 g/L)and organic solvent(mixture of cyclohexanol and dodecanol)as the porogenic agents

a novel hiperous matrix has been prepared by ultraviolet photo-polymerizing monomers of glycidyl methacrylate and ethylene glycol dimethacrylate by(W／O)／W emulsification．The matrix was modified with diethylamine to create an anion exchanger(DEA—B)．It was found that there was two families of pores (micropores and superpores)in DEA-B

but there was only microperes in the microporous beads(DEA—M)prepared bv O／W emulsification．The two families of pores in DEA-B were in the range of 50—200 nm and 500—5000 nm

respectively

while the pores of DEA—M were mainly in the range of 50—200 nm．The hydrated density and particle size of DEA—B were 1．08 g／mL and 46．3 μm

respectively

similar to those of DEA-M(1．10 g／mL and 45．2 μm)．Operated at a mobile phase velocity of 150 cm／h

the DEA-B column could be used to purify a 5．4 kb plasmid DNA．but the DEA-M columu failed to do that at the same condition．Then

HPLC pure plasmid DNA was were obtained with the DEA-B column operated at 1500 cm／h．The results indicated that DEA-B colulmn was promising for high-speed chromatography purification of plasmid DNA．