A kind of intramolecular charge transfer compound——3 methoxy 4' N

N dimethylamino flavone derivateves(DMMF) was used to detect collagen association in aqueous solution.Results showed that

when collagen concentration was increased to 0.5mg/mL or above

the fluorescence intensity of DMMF at 465nm increased dramatically.This indicated that collagen can associate in aqueous solution.A fluorescence polarization study on the same collagen solution also showed that the fluorescence polarization of DMMF rose rapidly when collagen concentration was increased to 0.5mg/mL or above

which proved that the system had high miecro-viscosity.This results also indicated that the aggregation behavior existed in the aqueous solution of collagen.