In order to increase structural selectivity

surface-initiated ATRP and ionic liquid (1-vinyl-3-ethyl acetate imidazolium chloride) as functional monomer were used to prepare molecularly imprinting membranes(MIMs).MIMs were prepared with lysozyme as template

ionic liquids (1-vinyl-3-ethyl acetate imidazolium chloride) as functional monomer

MBA as cross-linking agent

PMDETA as ligand

CuCl as catalyst through surface7-initiated ATRP on the initiator-functional regenerated cellulose membranes in aqueous solution at 35℃.The imprinted density and thickness of lysozyme molecular imprinted membranes were controlled by varying the concentrations of functional monomer.The imprinting factor reaches the maximum (6.25) at the ratio of functional monomer to initiator 100:1

with the water flux 83.0 L/m2/h.The equilibrium experimental data for the lysozyme-MIMs fitted the Langmuir isotherm well

and the binding amount reached 14.8 mg/g.The solid phase extraction experiments demonstrated that lysozyme-MIMs had specific separation and recognition performance in 4 min.Furthermore

permeability analysis experiments demonstrated that the lysozyme-MIMs had higher penetration ability for the target molecules than competitive molecules Cyt C and BHb.Based on the data obtained in separation of lysozyme from the mixed solution

the lysozyme-MIMs separation mechanism belongs to facilitated permeation

the template molecular lysozyme is first specifically adsorbed and concentrated

and then moves through the imprinted membrane by the binding/desorption process.